BMRB Entry 20092

Title:
To be advised
Deposition date:
2009-08-13
Original release date:
2010-10-13
Authors:
Fairlie, David; Harrison, Rosemary; Hoang, Huy; Ruiz-Gomez, Gloria
Citation:

Citation: Harrisona, Rosemary; Shepherda, Nicholas; Hoanga, Huy; Ruiz-Gomeza, Gloria; Hilla, Timothy; Drivera, Russell; Desaib, Vishal; Youngb, Paul; Abbenantea, Giovanni; Fairliea, David. "Downsizing human, bacterial, and viral proteins to short water-stable alpha helices that maintain biological potency"  Proc. Natl. Acad. Sci. U. S. A. 107, 11686-11691 (2010).
PubMed: 20543141

Assembly members:

Assembly members:
csp, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptococcus pneumoniae CSP-1   Taxonomy ID: 1313   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
csp: EXRLKKFFDKFILDRKKX

Data sets:
Data typeCount
1H chemical shifts89
coupling constants14

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1csp1

Entities:

Entity 1, csp 18 residues - Formula weight is not available

Resiude X represents Nle (Norleucine)

1   GLUNLEARGLEULYSLYSPHEPHEASPLYS
2   PHEILELEUASPARGLYSLYSNH2

Samples:

sample_1: csp2 – 5 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 4.1; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization

NMR spectrometers:

  • Bruker Avance 600 MHz