BMRB Entry 19869

Title:
ZapA mutant dimer from B. stearothermophilus
Deposition date:
2014-03-19
Original release date:
2015-06-15
Authors:
Nogueira, Maria Luiza; Sforca, Mauricio; Zeri, Ana
Citation:

Citation: Nogueira, Maria Luiza; Sforca, Mauricio; Zeri, Ana. "Zapa mutant dimer from B. stearothermophilus"  To be Published ., .-..

Assembly members:

Assembly members:
ZapA, polymer, 86 residues, 9902.521 Da.

Natural source:

Natural source:   Common Name: Geobacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: bacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts266
15N chemical shifts74
1H chemical shifts555

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chain A1
2Chain B1

Entities:

Entity 1, Chain A 86 residues - 9902.521 Da.

1   GLYSERHISMETTHRGLUGLNPROLYSTHR
2   ARGVALSERVALARGILETYRGLYGLNASP
3   TYRTHRILEVALGLYALAGLUSERPROALA
4   HISILEARGLEUVALALAALAPHEVALASP
5   ASPLYSMETHISGLUPHESERGLULYSGLN
6   PROMETLEUASPVALPROLYSLEUALAVAL
7   LEUTHRALAVALGLNILEALASERGLUTYR
8   LEULYSLEULYSGLUGLUTYRGLNARGLEU
9   ARGGLUGLNLEULYSLYS

Samples:

sample_1: ZapA, [U-15N], 0.3 mM; sodium phosphate 50 mM; potassium chloride 50 mM

sample_2: ZapA, [U-13C; U-15N], 0.3 mM; sodium phosphate 50 mM; potassium chloride 50 mM

sample_3: ZapA, [U-13C; U-15N], 0.3 mM; ZapA 0.3 mM; sodium phosphate 50 mM; potassium chloride 50 mM

conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicconditions_1
2D 13C-15N CHSQCsample_1isotropicconditions_1
3D 1H-13C NOESY aromaticsample_1isotropicconditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicconditions_1
3D CBCA(CO)NHsample_1isotropicconditions_1
3D HNCOsample_1isotropicconditions_1
3D HBHA(CO)NHsample_1isotropicconditions_1
3D HNCACBsample_1isotropicconditions_1
3D 13C-15N filtered NOESY C13 HSQCsample_3isotropicconditions_1
3D HCCH-TOCSYsample_1isotropicconditions_1
2D 1H-15N HSQCsample_3isotropicconditions_1
3D 15N - NOESY-HSQCsample_1isotropicconditions_1
3D 15N - TOCSY -HSQCsample_1isotropicconditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

SPARKY, Goddard - chemical shift assignment, geometry optimization, processing

NMR spectrometers:

  • BRUKER AVANCE 900 MHz
  • Agilent INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks