BMRB Entry 19663

Title:
Somatostatin-14 solution structure in 5% D-mannitol
Deposition date:
2013-12-08
Original release date:
2014-05-05
Authors:
Anoop, A.; Ranganathan, Srivastav; Pratihar, Supriya
Citation:

Citation: Anoop, Arunagiri; Ranganathan, Srivastav; Dhaked, Bhagwan Das; Jha, Narendra Nath; Pratihar, Supriya; Ghosh, Saikat; Sahay, Shruti; Kumar, Santosh; Das, Subhadeep; Kombrabail, Mamata; Agarwal, Kumud; Jacob, Reeba; Singru, Praful; Bhaumik, Prasenjit; Padinhateeri, Ranjith; Kumar, Ashutosh; Maji, Samir. "Elucidating the Role of Disulfide Bond on Amyloid Formation and Fibril Reversibility of Somatostatin-14: RELEVANCE TO ITS STORAGE AND SECRETION."  J. Biol. Chem. 289, 16884-16903 (2014).
PubMed: 24782311

Assembly members:

Assembly members:
Somatostatin-14, polymer, 14 residues, 1641.918 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Somatostatin-14: AGCKNFFWKTFTSC

Data sets:
Data typeCount
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Somatostatin-141

Entities:

Entity 1, Somatostatin-14 14 residues - 1641.918 Da.

1   ALAGLYCYSLYSASNPHEPHETRPLYSTHR
2   PHETHRSERCYS

Samples:

sample_1: H2O 90%; D2O 10%; D-mannitol 5 w/v %

sample_conditions_1: pH: 5.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY v3.114, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz