BMRB Entry 19590

Title:
Aliphatic 13C Chemical Shift Assignments For the Unfolded State of the Fyn SH3 Domain G48A Mutant
Deposition date:
2013-10-30
Original release date:
2014-02-11
Authors:
Bouvignies, Guillaume; Vallurupalli, Pramodh; Kay, Lewis
Citation:

Citation: Bouvignies, Guillaume; Vallurupalli, Pramodh; Kay, Lewis. "Visualizing side chains of invisible protein conformers by solution NMR."  J. Mol. Biol. 426, 763-774 (2014).
PubMed: 24211467

Assembly members:

Assembly members:
Fyn_SH3_G48A, polymer, 60 residues, 6921.4 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-29b(+)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fyn_SH3_G48A, 11
2Fyn_SH3_G48A, 21

Entities:

Entity 1, Fyn_SH3_G48A, 1 60 residues - 6921.4 Da.

1   SERTHRLEUPHEGLUALALEUTYRASPTYR
2   GLUALAARGTHRGLUASPASPLEUSERPHE
3   HISLYSGLYGLULYSPHEGLNILELEUASN
4   SERSERGLUGLYASPTRPTRPGLUALAARG
5   SERLEUTHRTHRGLYGLUTHRALATYRILE
6   PROSERASNTYRVALALAPROVALASPARG

Samples:

sample_1: Fyn_SH3_G48A, [U-100% 13C; U-100% 15N], 2.0 mM; sodium phosphate 50 mM; EDTA 0.2 mM; sodium azide 0.05%; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D aliphatic 13C CESTsample_1isotropicsample_conditions_1

Software:

NMRPipe v7.5 Rev 2012.204.11.07, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

UNP Q05876
EMBL CAA37025
SP Q05876
AlphaFold Q05876 Q05876