BMRB Entry 19570

Title:
Solution NMR structure of the cactus-derived antimicrobial peptide Ep-AMP1
Deposition date:
2013-10-22
Original release date:
2014-11-10
Authors:
Rosengren, K. Johan; Goransson, Ulf; Gunasekera, Sunithi; Aboye, Teshome
Citation:

Citation: Aboye, Teshome; Stromstedt, Adam; Bruhn, Jan; El-Seedi, Hesham; Gunasekera, Sunithi; Rosengren, K. Johan; Goransson, Ulf. "A cactus derived toxin-like cystine knot peptide with selective antimicrobial activity"  ChemBioChem ., .-..

Assembly members:

Assembly members:
Ep-AMP1, polymer, 35 residues, 3611.234 Da.

Natural source:

Natural source:   Common Name: eudicots   Taxonomy ID: 1001097   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Echinopsis pachanoi

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ep-AMP1: CVLIGQRCDNDRGPRCCSGQ GNCVPLPFLGGVCAV

Data sets:
Data typeCount
13C chemical shifts62
1H chemical shifts216

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cactus-derived antimicrobial peptide Ep-AMP11

Entities:

Entity 1, cactus-derived antimicrobial peptide Ep-AMP1 35 residues - 3611.234 Da.

1   CYSVALLEUILEGLYGLNARGCYSASPASN
2   ASPARGGLYPROARGCYSCYSSERGLYGLN
3   GLYASNCYSVALPROLEUPROPHELEUGLY
4   GLYVALCYSALAVAL

Samples:

sample_1: Ep-AMP1 6 mg/mL; H2O 90%; D2O 10%

sample_2: Ep-AMP1 4 mg/mL; D2O 100%

sample_conditions_1: ionic strength: 0 M; pH: 3.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H ECOSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB