BMRB Entry 19536

Title:
1H, 13C, 15N chemical shift assignments of Streptomyces virginiae VirA acp5a
Deposition date:
2013-10-05
Original release date:
2014-06-02
Authors:
Davison, Jack; Dorival, Jonathan; Rabeharindranto, M; Mazon, Hortense; Chagot, Benjamin; Gruez, Arnaud; Weissman, Kira
Citation:

Citation: Davison, Jack; Dorival, Jonathan; Rabeharindranto, M; Mazon, Hortense; Chagot, Benjamin; Gruez, Arnaud; Weissman, Kira. "NMR assignements of ACP5a"  .

Assembly members:

Assembly members:
entity, polymer, 85 residues, 8883.024 Da.

Natural source:

Natural source:   Common Name: Streptomyces virginiae   Taxonomy ID: 1961   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces virginiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBG102 (pET27 derivative)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts364
15N chemical shifts88
1H chemical shifts587

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1acp5a1

Entities:

Entity 1, acp5a 85 residues - 8883.024 Da.

1   GLYPROGLYSERALAGLYARGGLNGLUGLU
2   ILEALAGLUGLUVALALAARGLEULEUALA
3   GLYVALLEUTYRLEUGLUPROASPARGLEU
4   ASPPROGLUGLUTHRPHELEUTHRLEUGLY
5   VALASPSERILELEUGLYVALGLUPHEVAL
6   ALAALAVALASNALAALATYRPROVALGLY
7   VALLYSALATHRALALEUTYRASPHISPRO
8   THRPROALAALAPHEALAARGHISILEALA
9   GLUSERLEUGLYALA

Samples:

sample_1: sodium phosphate 100 mM; EDTA 1 mM; acp5a, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

AMBER v11, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks