BMRB Entry 19511

Title:
NMR Structure of the homeodomain transcription factor Gbx1 from Homo sapiens solved in the presence of the DNA sequence CGACTAATTAGTCG
Deposition date:
2013-09-20
Original release date:
2013-09-30
Authors:
Proudfoot, Andrew; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt
Citation:

Citation: Proudfoot, Andrew; Geralt, Michael; Elsliger, Marc-Andre; Wilson, Ian; Wuthrich, Kurt; Serrano, Pedro. "Dynamic Local Polymorphisms in the Gbx1 Homeodomain Induced by DNA Binding"  Structure 24, 1372-1379 (2016).
PubMed: 27396829

Assembly members:

Assembly members:
Gbx1, polymer, 71 residues, 8346.782 Da.
DNA_1, polymer, 14 residues, Formula weight is not available
DNA_2, polymer, 14 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET46

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts75
1H chemical shifts493

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeodomain transcription factor Gbx11
2DNA_12
3DNA_23

Entities:

Entity 1, homeodomain transcription factor Gbx1 71 residues - 8346.782 Da.

1   SERALAPROGLYGLYLYSSERARGARGARG
2   ARGTHRALAPHETHRSERGLUGLNLEULEU
3   GLULEUGLULYSGLUPHEHISCYSLYSLYS
4   TYRLEUSERLEUTHRGLUARGSERGLNILE
5   ALAHISALALEULYSLEUSERGLUVALGLN
6   VALLYSILETRPPHEGLNASNARGARGALA
7   LYSTRPLYSARGILELYSALAGLYASNVAL
8   SER

Entity 2, DNA_1 14 residues - Formula weight is not available

1   DCDGDADCDTDADADTDTDA
2   DGDTDCDG

Entity 3, DNA_2 14 residues - Formula weight is not available

1   DCDGDADCDTDADADTDTDA
2   DGDTDCDG

Samples:

sample_1: Gbx1, [U-98% 13C; U-98% 15N], 0.8 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; DNA_1 0.8 mM; DNA_2 0.8 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.220 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
APSY 4D-HACANHsample_1isotropicsample_conditions_1
APSY 5D-CBCACONHsample_1isotropicsample_conditions_1
APSY 5D-HACACONHsample_1isotropicsample_conditions_1

Software:

CYANA, G ntert P., Keller and Wuthrich - chemical shift assignment, refinement

Opalp, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

UNIO, Herrmann and Wuthrich - chemical shift assignment, structure solution

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks