BMRB Entry 19322

Title:
LTBP1 cbEGF14-TB3-EGF3
Deposition date:
2013-06-26
Original release date:
2014-02-13
Authors:
Robertson, Ian; Handford, Penny; Redfield, Christina
Citation:

Citation: Robertson, Ian; Handford, Penny; Redfield, Christina. "NMR Spectroscopic and Bioinformatic Analyses of the LTBP1 C-Terminus Reveal a Highly Dynamic Domain Organisation."  PLoS ONE 9, e87125-e87125 (2014).
PubMed: 24489852

Assembly members:

Assembly members:
cb14TB3, polymer, 117 residues, Formula weight is not available
TB3E3, polymer, 172 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Data sets:
Data typeCount
15N chemical shifts236
1H chemical shifts489

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cb14TB31
2TB3E32
3CALCIUM ION3

Entities:

Entity 1, cb14TB3 117 residues - Formula weight is not available

1   SERALAASPMETASPGLUCYSGLNASPPRO
2   SERSERCYSILEASPGLYGLNCYSVALASN
3   THRGLUGLYSERTYRASNCYSPHECYSTHR
4   HISPROMETVALLEUASPALASERGLULYS
5   ARGCYSILEARGPROALAGLUSERASNGLU
6   GLNILEGLUGLUTHRASPVALTYRGLNASP
7   LEUCYSTRPGLUHISLEUSERASPGLUTYR
8   VALCYSSERARGPROLEUVALGLYLYSGLN
9   THRTHRTYRTHRGLUCYSCYSCYSLEUTYR
10   GLYGLUALATRPGLYMETGLNCYSALALEU
11   CYSPROLEULYSASPSERASPASPTYRALA
12   GLNLEUCYSASNILEPROVAL

Entity 2, TB3E3 172 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERILEGLUGLYARGSERALAARGPRO
3   ALAGLUSERASNGLUGLNILEGLUGLUTHR
4   ASPVALTYRGLNASPLEUCYSTRPGLUHIS
5   LEUSERASPGLUTYRVALCYSSERARGPRO
6   LEUVALGLYLYSGLNTHRTHRTYRTHRGLU
7   CYSCYSCYSLEUTYRGLYGLUALATRPGLY
8   METGLNCYSALALEUCYSPROLEULYSASP
9   SERASPASPTYRALAGLNLEUCYSASNILE
10   PROVALTHRGLYARGARGGLNPROTYRGLY
11   ARGASPALALEUVALASPPHESERGLUGLN
12   TYRTHRPROGLUALAASPPROTYRPHEILE
13   GLNASPARGPHELEUASNSERPHEGLUGLU
14   LEUGLNALAGLUGLUCYSGLYILELEUASN
15   GLYCYSGLUASNGLYARGCYSVALARGVAL
16   GLNGLUGLYTYRTHRCYSASPCYSPHEASP
17   GLYTYRHISLEUASPTHRALALYSMETTHR
18   CYSVAL

Entity 3, CALCIUM ION - Ca - 40.078 Da.

1   CA

Samples:

sample_LTBP1-cb14TB3-1: cb14TB3, [U-100% 15N], 2 – 4 mM; TB3E3, [U-100% 15N], 2 – 4 mM; Calcium Chloride 20 mM

sample_LTBP1-cb14TB3-2: cb14TB3, [U-100% 15N], 2 – 3 mM; TB3E3, [U-100% 15N], 2 – 3 mM

sample_conditions-1: ionic strength: 60 mM; pH: 5.9; pressure: 1 atm; temperature: 273 K

sample_conditions-2: ionic strength: 0 mM; pH: 6.9; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_LTBP1-cb14TB3-1isotropicsample_conditions-1
3D 1H-15N NOESYsample_LTBP1-cb14TB3-1isotropicsample_conditions-1
3D 1H-15N TOCSYsample_LTBP1-cb14TB3-1isotropicsample_conditions-1
2D 1H-15N HSQCsample_LTBP1-cb14TB3-2isotropicsample_conditions-2
3D 1H-15N NOESYsample_LTBP1-cb14TB3-2isotropicsample_conditions-2
3D 1H-15N TOCSYsample_LTBP1-cb14TB3-2isotropicsample_conditions-2
3D 1H-15N HSQC-NOESY-HSQCsample_LTBP1-cb14TB3-1isotropicsample_conditions-1
3D 1H-15N HSQC-NOESY-HSQCsample_LTBP1-cb14TB3-2isotropicsample_conditions-2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Home built OMEGA 500 MHz
  • Home built OMEGA 600 MHz

Related Database Links:

UNP Q14766 Q14766
DBJ BAG61269
GB AAY15036
AlphaFold Q8TD95 Q8TD95

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks