BMRB Entry 19236
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19236
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Citation: Guan, Jia-Ying; Foester, Johannes; Drijfhout, Jan Wouter; Timmer, Monika; Blok, Anneloes; Ullmann, G. Matthias; Ubbink, Marcellus. "Dynamics in the complex of plastocyanin and tetralysine peptides" .
Assembly members:
plastocyanin, polymer, 102 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Ferns Taxonomy ID: 97234 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Dryopteris crassirhizoma
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28c
Entity Sequences (FASTA):
plastocyanin: AKVEVGDEVGNFKFYPDSIT
VSAGEAVEFTLVGETGHNIV
FDIPAGAPGTVASELKAASM
DENDLLSEDEPSFKAKVSTP
GTYTFYCTPHKSANMKGTLT
VK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 195 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | plastocyanin | 1 |
| 2 | zinc | 2 |
Entities:
Entity 1, plastocyanin 102 residues - Formula weight is not available
| 1 | ALA | LYS | VAL | GLU | VAL | GLY | ASP | GLU | VAL | GLY | ||||
| 2 | ASN | PHE | LYS | PHE | TYR | PRO | ASP | SER | ILE | THR | ||||
| 3 | VAL | SER | ALA | GLY | GLU | ALA | VAL | GLU | PHE | THR | ||||
| 4 | LEU | VAL | GLY | GLU | THR | GLY | HIS | ASN | ILE | VAL | ||||
| 5 | PHE | ASP | ILE | PRO | ALA | GLY | ALA | PRO | GLY | THR | ||||
| 6 | VAL | ALA | SER | GLU | LEU | LYS | ALA | ALA | SER | MET | ||||
| 7 | ASP | GLU | ASN | ASP | LEU | LEU | SER | GLU | ASP | GLU | ||||
| 8 | PRO | SER | PHE | LYS | ALA | LYS | VAL | SER | THR | PRO | ||||
| 9 | GLY | THR | TYR | THR | PHE | TYR | CYS | THR | PRO | HIS | ||||
| 10 | LYS | SER | ALA | ASN | MET | LYS | GLY | THR | LEU | THR | ||||
| 11 | VAL | LYS |
Entity 2, zinc - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: plastocyanin, [U-13C; U-15N], 2.4 ± 0.1 mM; sodium phosphate 10 mM
sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
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or all simulated peaks
SPARKY: Backbone
or all simulated peaks