BMRB Entry 19185

Title:
Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires of Geobacter sulfurreducens
Deposition date:
2013-04-22
Original release date:
2013-10-17
Authors:
Reardon, Patrick; Mueller, Karl
Citation:

Citation: Reardon, Patrick; Mueller, Karl. "Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires of Geobacter sulfurreducens"  .

Assembly members:

Assembly members:
PilA, polymer, 61 residues, 6576.573 Da.

Natural source:

Natural source:   Common Name: d-proteobacteria   Taxonomy ID: 35554   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacter sulfurreducens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTCLE

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts221
15N chemical shifts58
1H chemical shifts396

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PilA1

Entities:

Entity 1, PilA 61 residues - 6576.573 Da.

1   PHETHRLEUILEGLULEULEUILEVALVAL
2   ALAILEILEGLYILELEUALAALAILEALA
3   ILEPROGLNPHESERALATYRARGVALLYS
4   ALATYRASNSERALAALASERSERASPLEU
5   ARGASNLEULYSTHRALALEUGLUSERALA
6   PHEALAASPASPGLNTHRTYRPROPROGLU
7   SER

Samples:

sample_1: PilA, [U-13C; U-15N], 1 mM; DHPC, [U-2H], 200 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

VNMRJ v3.2, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

CARA, Keller and Wuthrich - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian VNMRs 800 MHz
  • Varian VNMRs 750 MHz

Related Database Links:

PDB
GB AAR34870 ADI84335
REF NP_952547 WP_010942142 WP_049786599

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks