BMRB Entry 18922

Title:
1H, 13C and 15N assignments of the dimeric receiver domain of PmrAn response regulator from Klebsiella pneumonia
Deposition date:
2012-12-29
Original release date:
2014-04-22
Authors:
Luo, Shih-chi
Citation:

Citation: Luo, Shih-Chi; Lou, Yuan-Chao; Rajasekaran, Mahalingam; Chang, Yi-Wei; Hsiao, Chwan-Deng; Chen, Chinpan. "Structural basis of a physical blockage mechanism for the interaction of response regulator PmrA with connector protein PmrD from Klebsiella pneumoniae."  J. Biol. Chem. 288, 25551-25561 (2013).
PubMed: 23861396

Assembly members:

Assembly members:
PmrAn, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Klebsiella pneumoniae   Taxonomy ID: 573   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Klebsiella pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Data sets:
Data typeCount
13C chemical shifts352
15N chemical shifts117
1H chemical shifts428

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PmrAn, subunit 11
2PmrAn, subunit 21

Entities:

Entity 1, PmrAn, subunit 1 123 residues - Formula weight is not available

1   METLYSILELEUVALILEGLUASPASPALA
2   LEULEULEUGLNGLYLEUILELEUALAMET
3   GLNSERGLUGLYTYRVALCYSASPGLYVAL
4   SERTHRALAHISGLUALAALALEUSERLEU
5   ALASERASNHISTYRSERLEUILEVALLEU
6   ASPLEUGLYLEUPROASPGLUASPGLYLEU
7   HISPHELEUSERARGMETARGARGGLULYS
8   METTHRGLNPROVALLEUILELEUTHRALA
9   ARGASPTHRLEUGLUASPARGILESERGLY
10   LEUASPTHRGLYALAASPASPTYRLEUVAL
11   LYSPROPHEALALEUGLUGLULEUASNALA
12   ARGILEARGALALEULEUARGARGHISASN
13   ASNGLNGLY

Samples:

sample_1: PmrAn, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 – 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 850 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 26532 26535
PDB
DBJ BAH62495 BAS36450
EMBL CCI78009 CCM81779 CCM90377 CCM95353 CCN28862
GB ABR76243 ACI11257 ADC59454 AEJ97287 AEW60332
REF WP_002895659 WP_004142095 WP_004886130 WP_008805743 WP_016947497

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks