BMRB Entry 18828

Title:
NMR Assignment of Arabidopsis thaliana cytochrome c in its reduced state
Deposition date:
2012-11-07
Original release date:
2014-02-14
Authors:
Moreno-Beltran, Blas; Diaz-Moreno, Irene; Diaz-Quintana, Antonio; De la Rosa, Miguel
Citation:

Citation: Hervas, Manuel; Bashir, Qamar; Leferink, Nicole; Ferreira, Patricia; Moreno-Beltran, Blas; Westphal, Adrie; Diaz-Moreno, Irene; Medina, Milagros; de la Rosa, Miguel; Ubbink, Marcellus; Navarro, Jose; van Berkel, Willem. "Communication between (L)-galactono-1,4-lactone dehydrogenase and cytochrome c."  FEBS J. 280, 1830-1840 (2013).
PubMed: 23438074

Assembly members:

Assembly members:
cytochrome_c_peptide, polymer, 111 residues, Formula weight is not available
HEME C, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBTR_AtCc

Data sets:
Data typeCount
15N chemical shifts112
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c1
2HEC2

Entities:

Entity 1, cytochrome c 111 residues - Formula weight is not available

1   ALASERPHEASPGLUALAPROPROGLYASN
2   PROLYSALAGLYGLULYSILEPHEARGTHR
3   LYSCYSALAGLNCYSHISTHRVALGLULYS
4   GLYALAGLYHISLYSGLNGLYPROASNLEU
5   ASNGLYLEUPHEGLYARGGLNSERGLYTHR
6   THRPROGLYTYRSERTYRSERALAALAASN
7   LYSSERMETALAVALASNTRPGLUGLULYS
8   THRLEUTYRASPTYRLEULEUASNPROLYS
9   LYSTYRILEPROGLYTHRLYSMETVALPHE
10   PROGLYLEULYSLYSPROGLNASPARGALA
11   ASPLEUILEALATYRLEULYSGLUGLYTHR
12   ALA

Entity 2, HEC - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

AtCc_sample: polymer, [U-99% 15N], 2 mM; sodium phosphate 5 mM; H2O 90%; D2O 10%

AtCc_cond: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCAtCc_sampleisotropicAtCc_cond
3D 1H-15N NOESYAtCc_sampleisotropicAtCc_cond
3D 1H-15N TOCSYAtCc_sampleisotropicAtCc_cond

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

EMBL CAB39628 CAB78127 CDX90744
GB AAK76618 AAL85104 AAM64617 AEE82832 EFH48744
REF NP_192742 XP_002872485 XP_006288926 XP_006397109 XP_009134179
SP Q9T0G2
AlphaFold Q9T0G2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks