BMRB Entry 18491

Title:
1H chemical shifts of Thermolysin 255-316 fragment
Deposition date:
2012-05-30
Original release date:
2012-06-18
Authors:
Rico, Manuel; Jimenez, M Angeles; Gonzalez, Carlos
Citation:

Citation: Rico, Manuel; Jimenez, M Angeles; Gonzalez, Carlos; de Filippis, Vincenzo; Fontana, Angelo. "NMR solution structure of the C-terminal fragment 255-316 of Thermolysin: A dimer formed by subunits having native structure"  Biochemistry 33, 14834-14847 (1994).
PubMed: 7993910

Assembly members:

Assembly members:
Th255-316, polymer, 62 residues, 6624.5 Da.

Natural source:

Natural source:   Common Name: Bacillus thermoproteolyticus   Taxonomy ID: 1427   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus thermoproteolyticus

Experimental source:

Experimental source:   Production method: Subtilisin limited proteolysis

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts448

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Th255-316, chain 11
2Th255-316, chain 21

Entities:

Entity 1, Th255-316, chain 1 62 residues - 6624.5 Da.

1   VALVALGLYILEGLYARGASPLYSLEUGLY
2   LYSILEPHETYRARGALALEUTHRGLNTYR
3   LEUTHRPROTHRSERASNPHESERGLNLEU
4   ARGALAALAALAVALGLNSERALATHRASP
5   LEUTYRGLYSERTHRSERGLNGLUVALALA
6   SERVALLYSGLNALAPHEASPALAVALGLY
7   VALLYS

Samples:

sample_1: Th255-316 1 mM; TSP 0.1 mM; H2O 90%; D2P 10%

sample_2: Th255-316 1 mM; TSP 0.1 mM; D2O 100%

sample_conditions_1: ionic strength: 0 M; pH: 4.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

UXNMR, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 18495
PDB
EMBL CAA54291
GB AAA22625 AAB02774
SP P00800 P43133
AlphaFold P43133 P00800