BMRB Entry 18331

Title:
1H, 13C and 15N assignments of the holo-acyl carrier protein of Pseudomonas aeruginosa
Deposition date:
2012-03-15
Original release date:
2012-09-14
Authors:
Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei
Citation:

Citation: Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei. "1H, 13C and 15N assignments of the holo-acyl carrier protein of Pseudomonas aeruginosa."  Biomol. NMR Assignments 7, 225-228 (2013).
PubMed: 22843382

Assembly members:

Assembly members:
PaAcpP, polymer, 81 residues, 9008.8515 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-14b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts338
15N chemical shifts83
1H chemical shifts563

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PaAcpP1

Entities:

Entity 1, PaAcpP 81 residues - 9008.8515 Da.

1   GLYSERHISMETSERTHRILEGLUGLUARG
2   VALLYSLYSILEVALALAGLUGLNLEUGLY
3   VALLYSGLUGLUGLUVALTHRASNSERALA
4   SERPHEVALGLUASPLEUGLYALAASPSER
5   LEUASPTHRVALGLULEUVALMETALALEU
6   GLUGLUGLUPHEGLUTHRGLUILEPROASP
7   GLULYSALAGLULYSILETHRTHRVALGLN
8   GLUALAILEASPTYRILEVALALAHISGLN
9   GLN

Samples:

15N-labelled: PaAcpP, [U-99% 15N], 2.0 mM; sodium phosphate 50.0 mM; sodium chloride 50.0 mM; H2O 5%; D2O 95%

13C-15N-labelled: PaAcpP, [U-99% 13C; U-99% 15N], 2.0 mM; sodium phosphate 50.0 mM; sodium chloride 50.0 mM; H2O 5%; D2O 95%

13C-15N_D2O: PaAcpP, [U-99% 13C; U-99% 15N], 2.0 mM; sodium phosphate 50.0 mM; sodium chloride 50.0 mM; D2O 100%

CondSet1: ionic strength: 0.050 M; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQC15N-labelledisotropicCondSet1
3D HNCACB13C-15N-labelledisotropicCondSet1
cbcaconh ({CA|Cca}coNH)13C-15N-labelledisotropicCondSet1
3D HNCO13C-15N-labelledisotropicCondSet1
hCCconh (C_ccoNH.TOCSY)13C-15N-labelledisotropicCondSet1
3D 1H-15N NOESY15N-labelledisotropicCondSet1
3D H(CCO)NH13C-15N-labelledisotropicCondSet1
2D 1H-13C HSQC/HMQC13C-15N_D2OisotropicCondSet1
hbCBcgcdHD13C-15N_D2OisotropicCondSet1
HMBC-HSQC13C-15N_D2OisotropicCondSet1

Software:

ANALYSIS v2.1, CCPN - assignment

TALOS+, Shen Yang - secondary structure prediction

TOPSPIN v2.1, Bruker - acquisition

NMRPipe v2011, Frank Delaglio - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 18821
DBJ BAK90915 BAP22431 BAP50057 BAQ38943 BAR67004
EMBL CAW26824 CCQ85094 CDF85073 CDH70383 CDH76426
GB AAB94392 AAG06354 ABJ12204 ABR81030 AEO74481
REF NP_251656 WP_003091135 WP_007160597 WP_015477931
SP O54439
AlphaFold O54439

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks