BMRB Entry 18308

Title:
Backbone amide assignments of HLA-DR1/CLIP complexes
Deposition date:
2012-03-01
Original release date:
2019-09-13
Authors:
Schlundt, Andreas; Guenther, Sebastian; Sticht, Jana; Wieczorek, Marek; Roske, Yvette; Heinemann, Udo; Freund, Christian
Citation:

Citation: Schlundt, Andreas; Guenther, Sebastian; Sticht, Jana; Wieczorek, Marek; Roske, Yvette; Heinemann, Udo; Freund, Christian. "Peptide linkage to the alpha-subunit of MHCII creates a stably inverted antigen presentation complex"  J. Mol. Biol. 423, 294-302 (2012).
PubMed: 22820093

Assembly members:

Assembly members:
HLA-DR1_alpha_subunit, polymer, 193 residues, Formula weight is not available
HLA-DR1_beta_subunit, polymer, 199 residues, Formula weight is not available
CLIP106-120flipped, polymer, 15 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET system

Data sets:
Data typeCount
15N chemical shifts157
1H chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HLA-DR1_alpha_subunit1
2HLA-DR1_beta_subunit2
3CLIP106-1203

Entities:

Entity 1, HLA-DR1_alpha_subunit 193 residues - Formula weight is not available

1   METILELYSGLUGLUHISVALILEILEGLN
2   ALAGLUPHETYRLEUASNPROASPGLNSER
3   GLYGLUPHEMETPHEASPPHEASPGLYASP
4   GLUILEPHEHISVALASPMETALALYSLYS
5   GLUTHRVALTRPARGLEUGLUGLUPHEGLY
6   ARGPHEALASERPHEGLUALAGLNGLYALA
7   LEUALAASNILEALAVALASPLYSALAASN
8   LEUGLUILEMETTHRLYSARGSERASNTYR
9   THRPROILETHRASNVALPROPROGLUVAL
10   THRVALLEUTHRASNSERPROVALGLULEU
11   ARGGLUPROASNVALLEUILECYSPHEILE
12   ASPLYSPHETHRPROPROVALVALASNVAL
13   THRTRPLEUARGASNGLYLYSPROVALTHR
14   THRGLYVALSERGLUTHRVALPHELEUPRO
15   ARGGLUASPHISLEUPHEARGLYSPHEHIS
16   TYRLEUPROPHELEUPROSERTHRGLUASP
17   VALTYRASPCYSARGVALGLUHISTRPGLY
18   LEUASPGLUPROLEULEULYSHISTRPGLU
19   PHEASPALAPROSERPROLEUPROGLUTHR
20   THRGLUASN

Entity 2, HLA-DR1_beta_subunit 199 residues - Formula weight is not available

1   METGLYASPTHRARGPROARGPHELEUTRP
2   GLNLEULYSPHEGLUCYSHISPHEPHEASN
3   GLYTHRGLUARGVALARGLEULEUGLUARG
4   CYSILETYRASNGLNGLUGLUSERVALARG
5   PHEASPSERASPVALGLYGLUTYRARGALA
6   VALTHRGLULEUGLYARGPROASPALAGLU
7   TYRTRPASNSERGLNLYSASPLEULEUGLU
8   GLNARGARGALAALAVALASPTHRTYRCYS
9   ARGHISASNTYRGLYVALGLYGLUSERPHE
10   THRVALGLNARGARGVALGLUPROLYSVAL
11   THRVALTYRPROSERLYSTHRGLNPROLEU
12   GLNHISHISASNLEULEUVALCYSSERVAL
13   SERGLYPHETYRPROGLYSERILEGLUVAL
14   ARGTRPPHEARGASNGLYGLNGLUGLULYS
15   ALAGLYVALVALSERTHRGLYLEUILEGLN
16   ASNGLYASPTRPTHRPHEGLNTHRLEUVAL
17   METLEUGLUTHRVALPROARGSERGLYGLU
18   VALTYRTHRCYSGLNVALGLUHISPROSER
19   VALTHRSERPROLEUTHRVALGLUTRPARG
20   ALAARGSERGLUSERALAGLNSERLYS

Entity 3, CLIP106-120 15 residues - Formula weight is not available

class II-associated invariant chain-derived peptide

1   LYSMETARGMETALATHRPROLEULEUMET
2   GLNALALEUPROMET

Samples:

Sample_DRbeta_CLIP106-120: HLA-DR1_alpha_subunit 280-360 uM; HLA-DR1_beta_subunit, [U-13C; U-15N; U-2H], 280-360 uM; CLIP106-120 280-360 uM

sample_conditions_1: ionic strength: 150 mM; pH: 5.8; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCSample_DRbeta_CLIP106-120isotropicsample_conditions_1
3D HNCASample_DRbeta_CLIP106-120isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks