BMRB Entry 17816

Title:
lactococcin 972
Deposition date:
2011-07-29
Original release date:
2012-07-30
Authors:
Turner, David; Lamosa, Pedro; Martinez, Beatriz
Citation:

Citation: Turner, David; Lamosa, Pedro; Martinez, Beatriz. "Structure and properties of lactococcin 972 from Lactococcus lactis"  .

Assembly members:

Assembly members:
Lcn972, polymer, 66 residues, 7392.028 Da.

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Lactococcus lactis

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts423

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lcn9721

Entities:

Entity 1, Lcn972 66 residues - 7392.028 Da.

1   GLUGLYTHRTRPGLNHISGLYTYRGLYVAL
2   SERSERALATYRSERASNTYRHISHISGLY
3   SERLYSTHRHISSERALATHRVALVALASN
4   ASNASNTHRGLYARGGLNGLYLYSASPTHR
5   GLNARGALAGLYVALTRPALALYSALATHR
6   VALGLYARGASNLEUTHRGLULYSALASER
7   PHETYRTYRASNPHETRP

Samples:

sample_1: Lcn972 1.3 ± 0.1 mM; H2O 90 ± 0.1 %; D2O 10 ± 0.1 %

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, peak picking

TOPSPIN v3.0, Bruker Biospin - collection, processing

DYANA vINDYANA extended, Guntert, Braun and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CAA05247
GB AAF64054
REF NP_862432 WP_011117133 YP_009076498