BMRB Entry 17782

Title:
lsm57
Deposition date:
2011-07-15
Original release date:
2011-10-25
Authors:
Sprangers, Remco; Mund, Markus; N., Ancilla
Citation:

Citation: Mund, Markus; Neu, Ancilla; Ullmann, Janina; Neu, Ursula; Sprangers, Remco. "Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings."  J. Mol. Biol. 414, 165-176 (2011).
PubMed: 22001694

Assembly members:

Assembly members:
lsm57, polymer, 193 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet

Data sets:
Data typeCount
13C chemical shifts274
15N chemical shifts133
1H chemical shifts133

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lsm571

Entities:

Entity 1, lsm57 193 residues - Formula weight is not available

1   METSERMETTHRILELEUPROLEUGLULEU
2   ILEASPLYSCYSILEGLYSERASNLEUTRP
3   VALILEMETLYSSERGLUARGGLUPHEALA
4   GLYTHRLEUVALGLYPHEASPASPTYRVAL
5   ASNILEVALLEULYSASPVALTHRGLUTYR
6   ASPTHRVALTHRGLYVALTHRGLULYSHIS
7   SERGLUMETLEULEUASNGLYASNGLYMET
8   CYSMETLEUILEPROGLYGLYLYSPROGLU
9   METSERSERLEUGLNLYSARGPROGLYPRO
10   GLYASNSERSERGLNPROTHRGLUARGPRO
11   ARGLYSGLUSERILELEUASPLEUSERARG
12   TYRGLNASPGLNARGILEGLNALATHRPHE
13   THRGLYGLYARGGLNILETHRGLYILELEU
14   LYSGLYPHEASPGLNLEUMETASNLEUVAL
15   LEUASPASPVALGLUGLUGLNLEUARGASN
16   PROGLUASPGLYLYSLEUTHRGLYALAILE
17   ARGLYSLEUGLYLEUVALVALVALARGGLY
18   THRTHRLEUVALLEUILEALAPROMETASP
19   GLYSERGLUGLUILEPROASNPROPHEVAL
20   GLNALAGLU

Samples:

sample_1: lsm57, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mM; H2O 95%; D2O 5%; NaPO4 pH 7.5 50 mM; NaCl 150 mM; DTT 1 mM; NaN3 0.3%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAA20851
REF NP_588340
SP O74499
AlphaFold O74499

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks