BMRB Entry 17733

Title:
Solution structure of (R7G)-Crp4
Deposition date:
2011-06-26
Original release date:
2012-05-09
Authors:
Rosengren, K. Johan; Andersson, Hakan; Haugaard-Kedstrom, Linda; Bengtsson, Elina; Daly, Norelle; Figueredo, Sharel; Qu, Xiaoqing; Craik, David; Ouellette, Andre
Citation:

Citation: Andersson, Hakan; Figueredo, Sharel; Haugaard-Kedstrom, Linda; Bengtsson, Elina; Daly, Norelle; Qu, Xiaoqing; Craik, David; Ouellette, Andre; Rosengren, K. Johan. "The -defensin salt-bridge induces backbone stability to facilitate folding and confer proteolytic resistance."  Amino Acids 43, 1471-1483 (2012).
PubMed: 22286872

Assembly members:

Assembly members:
(R7G)-Crp4, polymer, 32 residues, 3671.471 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
(R7G)-Crp4: GLLCYCGKGHCKRGERVRGT CGIRFLYCCPRR

Data sets:
Data typeCount
1H chemical shifts188

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1(R7G)-Crp41

Entities:

Entity 1, (R7G)-Crp4 32 residues - 3671.471 Da.

1   GLYLEULEUCYSTYRCYSGLYLYSGLYHIS
2   CYSLYSARGGLYGLUARGVALARGGLYTHR
3   CYSGLYILEARGPHELEUTYRCYSCYSPRO
4   ARGARG

Samples:

sample_1: (R7G)-Crp4 1 mg; H2O 90%; D2O 10%

sample_conditions_1: pH: 4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB