BMRB Entry 17690

Title:
N-terminal regulatory segment of carnitine palmitoyltransferase 1A
Deposition date:
2011-06-06
Original release date:
2011-10-26
Authors:
Ulmer, Tobias; Rao, Jampani
Citation:

Citation: Rao, Jampani; Warren, Gemma; Estolt-Povedano, Sara; Zammit, Victor; Ulmer, Tobias. "An environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A."  J. Biol. Chem. 286, 42545-42554 (2011).
PubMed: 21990363

Assembly members:

Assembly members:
carnitine palmitoyltransferase 1A, polymer, 42 residues, 4802.562 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET44

Entity Sequences (FASTA):

Entity Sequences (FASTA):
carnitine palmitoyltransferase 1A: MAEAHQAVAFQFTVTPDGID LRLSHEALRQIYLSGLHSWK KK

Data sets:
Data typeCount
13C chemical shifts112
15N chemical shifts40
1H chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1carnitine palmitoyltransferase 1A1

Entities:

Entity 1, carnitine palmitoyltransferase 1A 42 residues - 4802.562 Da.

1   METALAGLUALAHISGLNALAVALALAPHE
2   GLNPHETHRVALTHRPROASPGLYILEASP
3   LEUARGLEUSERHISGLUALALEUARGGLN
4   ILETYRLEUSERGLYLEUHISSERTRPLYS
5   LYSLYS

Samples:

sample_1: palmitoyltransferase_1A, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; palmitoyltransferase_1A, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 95%; D2O 5%; MES+NaOH 25 mM; dodecytrimethylammonium chloride 150 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 5.6; pressure: 1 atm; temperature: 308.2 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
quant j correlationsample_1isotropicsample_conditions_1
3D NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAG36955
EMBL CAC80073 CAD12625 CAD59673
GB AAC41748 AAH00185 AAK69171 AAP88793 AAX32217
PRF 2108402A
REF NP_001009414 NP_001027017 NP_001123277 NP_001273789 NP_001291918
SP P50416
TPG DAA13488
AlphaFold P50416

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks