BMRB Entry 17631

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for human JAZ ZF2
Deposition date:
2011-05-08
Original release date:
2014-02-24
Authors:
Burge, Russell; Martinez-Yamout, Maria; Gottesfeld, Joel; Dyson, H.; Wright, Peter; Dyson, Jane
Citation:

Citation: Burge, Russell; Martinez-Yamout, Maria; Gottesfeld, Joel; Dyson, H.; Wright, Peter. "Backbone assignment of human JAZ ZF2"  Biochemistry ., .-..

Assembly members:

Assembly members:
JAZ_ZF2, polymer, 55 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts80
15N chemical shifts45
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1JAZ ZF21
2zinc ion2

Entities:

Entity 1, JAZ ZF2 55 residues - Formula weight is not available

1   GLYGLUTHRLYSLYSLEUASPSERASPGLN
2   LYSSERSERARGSERLYSASPLYSASNGLN
3   CYSCYSPROILECYSASNMETTHRPHESER
4   SERPROVALVALALAGLNSERHISTYRLEU
5   GLYLYSTHRHISALALYSASNLEULYSLEU
6   LYSGLNGLNSERTHR

Entity 2, zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: JAZ ZF2, [U-98% 13C; U-98% 15N], 300 uM; TRIS 25 mM; potassium chloride 50 mM; D2O, [U-99% 2H], 7%; DTT 2 mM; zinc 50 uM; H2O 90%

sample_conditions_1: ionic strength: 75 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView v5, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

GB EAW85038 AAD52018 AAH07775 AIC50988 EAW85038 EAW85040
DBJ BAD18614 BAG72878
EMBL CAH18475 CAL37677 CAL38643
REF NP_001231409 NP_001295142 NP_001295144 NP_036411 XP_001498579
SP Q9UL40
AlphaFold Q9UL40

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks