BMRB Entry 17597

Title:
Chemical shift assignments for Rv0577, a putative glyoxylase associated with virulence from Mycobacterium tuberculosis. Seattle Structural Genomics Center for Infectious Disease (SSGCID) target MytuD.17269.a.
Deposition date:
2011-04-21
Original release date:
2011-05-19
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Kim, Heungbok; Myler, Peter; Terwilliger, Thomas; Kim, Chang-Yub. "Chemical shift assignments for Rv0577, a putative glyoxylase associated with virulence from Mycobacterium tuberculosis."  Biomol. NMR Assignments 6, 43-46 (2012).
PubMed: 21761124

Assembly members:

Assembly members:
Rv0577, polymer, 269 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMCSG7

Data sets:
Data typeCount
13C chemical shifts772
15N chemical shifts221
1H chemical shifts758

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rv05771

Entities:

Entity 1, Rv0577 269 residues - Formula weight is not available

1   METPROLYSARGSERGLUTYRARGGLNGLY
2   THRPROASNTRPVALASPLEUGLNTHRTHR
3   ASPGLNSERALAALALYSLYSPHETYRTHR
4   SERLEUPHEGLYTRPGLYTYRASPASPASN
5   PROVALPROGLYGLYGLYGLYVALTYRSER
6   METALATHRLEUASNGLYGLUALAVALALA
7   ALAILEALAPROMETPROPROGLYALAPRO
8   GLUGLYMETPROPROILETRPASNTHRTYR
9   ILEALAVALASPASPVALASPALAVALVAL
10   ASPLYSVALVALPROGLYGLYGLYGLNVAL
11   METMETPROALAPHEASPILEGLYASPALA
12   GLYARGMETSERPHEILETHRASPPROTHR
13   GLYALAALAVALGLYLEUTRPGLNALAASN
14   ARGHISILEGLYALATHRLEUVALASNGLU
15   THRGLYTHRLEUILETRPASNGLULEULEU
16   THRASPLYSPROASPLEUALALEUALAPHE
17   TYRGLUALAVALVALGLYLEUTHRHISSER
18   SERMETGLUILEALAALAGLYGLNASNTYR
19   ARGVALLEULYSALAGLYASPALAGLUVAL
20   GLYGLYCYSMETGLUPROPROMETPROGLY
21   VALPROASNHISTRPHISVALTYRPHEALA
22   VALASPASPALAASPALATHRALAALALYS
23   ALAALAALAALAGLYGLYGLNVALILEALA
24   GLUPROALAASPILEPROSERVALGLYARG
25   PHEALAVALLEUSERASPPROGLNGLYALA
26   ILEPHESERVALLEULYSPROALAPROGLN
27   GLNARGSERHISHISHISHISHISHIS

Samples:

sample_1: Rv0577, [U-99% 13C; U-99% 15N], 1.5 ± 0.2 mM; sodium chloride 100 ± 5 mM; TRIS 20 ± 2 mM; DTT 1 ± 0.2 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.1; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D HBCBCGCDHDsample_1isotropicsample_conditions_1
2D HBCBCGCDCHEsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - data analysis

FELIX v2007, Accelrys Software Inc. - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAH24886 BAL64448 BAQ04462 GAA44359
EMBL CAA07636 CAL70607 CCC25656 CCC63181 CCE36117
GB AAK44829 ABQ72307 ABR04930 ACJ14499 ACJ14500
REF NP_215091 NP_854252 WP_003403012 WP_015287862 WP_015292278
SP P0A5N9 P9WIR2 P9WIR3
AlphaFold P0A5N9 P9WIR3 P9WIR2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks