BMRB Entry 17286

Title:
Solution structure of the C-terminal domain of hRpn13
Deposition date:
2010-11-08
Original release date:
2012-07-25
Authors:
Zhou, Zi-ren; Hu, Hong-yu
Citation:

Citation: Zhou, Zi-ren; Hu, Hong-yu. "Solution structure of the C-terminal domain of hRpn13"  .

Assembly members:

Assembly members:
entity, polymer, 150 residues, 15804.739 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-MG

Data sets:
Data typeCount
13C chemical shifts536
15N chemical shifts130
1H chemical shifts773

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hRpn131

Entities:

Entity 1, hRpn13 150 residues - 15804.739 Da.

1   GLYSERGLNPROILEGLNLEUSERASPLEU
2   GLNSERILELEUALATHRMETASNVALPRO
3   ALAGLYPROALAGLYGLYGLNGLNVALASP
4   LEUALASERVALLEUTHRPROGLUILEMET
5   ALAPROILELEUALAASNALAASPVALGLN
6   GLUARGLEULEUPROTYRLEUPROSERGLY
7   GLUSERLEUPROGLNTHRALAASPGLUILE
8   GLNASNTHRLEUTHRSERPROGLNPHEGLN
9   GLNALALEUGLYMETPHESERALAALALEU
10   ALASERGLYGLNLEUGLYPROLEUMETCYS
11   GLNPHEGLYLEUPROALAGLUALAVALGLU
12   ALAALAASNLYSGLYASPVALGLUALAPHE
13   ALALYSALAMETGLNASNASNALALYSPRO
14   GLUGLNLYSGLUGLYASPTHRLYSASPLYS
15   LYSASPGLUGLUGLUASPMETSERLEUASP

Samples:

sample_1: hRpn13 20 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.075 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-COSYsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1
3D 1H-15N NOESY-HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY-HSQCsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19801
PDB
DBJ BAA11023 BAJ20420
EMBL CAH18070
GB AAH10733 AAH17245 AAX42432 ABM84246 ABM87634
REF NP_001247458 NP_001268366 NP_001268367 NP_008933 NP_783163
SP Q16186
TPD FAA00246
AlphaFold Q16186

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks