BMRB Entry 17036

Title:
SOLUTION STRUCTURE OF ZINC FINGER DOMAIN OF E3 UBIQUITIN-PROTEIN LIGASE PROTEIN Praja-1 FROM HOMO SAPIENS, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET HR4710B
Deposition date:
2010-06-30
Original release date:
2010-08-24
Authors:
Liu, Gaohua; Tong, Sai; Hamilton, Keith; Ciccosanti, Colleen; Shastry, Ritu; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano
Citation:

Citation: Liu, Gaohua; Tong, Sai; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano. "SOLUTION STRUCTURE OF ZINC FINGER DOMAIN OF E3 UBIQUITIN-PROTEIN LIGASE PROTEIN Praja-1 FROM HOMO SAPIENS, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET HR4710B"  .

Assembly members:

Assembly members:
pja1_zinc_finger_domain, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 14-15C

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts277
15N chemical shifts73
1H chemical shifts554

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pja1 zinc finger domain1

Entities:

Entity 1, pja1 zinc finger domain 91 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISSERHIS
2   METVALALAASNPROPROALASERLYSGLU
3   SERILEASPALALEUPROGLUILELEUVAL
4   THRGLUASPHISGLYALAVALGLYGLNGLU
5   METCYSCYSPROILECYSCYSSERGLUTYR
6   VALLYSGLYASPVALALATHRGLULEUPRO
7   CYSHISHISTYRPHEHISLYSPROCYSVAL
8   SERILETRPLEUGLNLYSSERGLYTHRCYS
9   PROVALCYSARGCYSMETPHEPROPROPRO
10   LEU

Samples:

sample_NC: pja1 zinc finger domain, [U-100% 13C; U-100% 15N], 0.94 mM; H2O 90%; D2O 10%

sample_nc5: pja1 zinc finger domain, [U-10% 13C; U-100% 15N], 0.82 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_NCisotropicsample_conditions_1
2D 1H-13C HSQCsample_nc5isotropicsample_conditions_1
3D 1H-13C-15N SIMUTANEOUS NOESYsample_NCisotropicsample_conditions_1
3D 1H-13C aromatic NOESYsample_NCisotropicsample_conditions_1
3D HNCACBsample_NCisotropicsample_conditions_1
3D CBCA(CO)NHsample_NCisotropicsample_conditions_1
3D C(CO)NHsample_NCisotropicsample_conditions_1
3D HNCOsample_NCisotropicsample_conditions_1
3D HCCH-TOCSYsample_NCisotropicsample_conditions_1
3D HBHA(CO)NHsample_NCisotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinement, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution

AutoStruct, Huang, Tejero, Powers and Montelione - data analysis, geometry optimization, refinement

TALOS+, Shen, Delaglio and Bax - data analysis

XEASY, Bartels et al. - data analysis, peak picking, refinement

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB13928 BAB23982 BAE22512 BAE73073 BAE88506
EMBL CAH93231
GB AAC00205 AAH08148 AAH25975 AAH37616 AAH48323
REF NP_001027568 NP_001076579 NP_001094476 NP_001128898 NP_001277484
SP O55176 Q8NG27
AlphaFold O55176 Q8NG27

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks