BMRB Entry 1696

Title:
Complete 1H and 13C assignment of Lys and Leu sidechains of staphylococcal nuclease using HCCH-COSY and HCCH-TOCSY 3D NMR spectroscopy
Deposition date:
1995-07-31
Original release date:
1995-07-31
Authors:
Baldisseri, Donna; Pelton, J.; Sparks, Steven; Torchia, Dennis
Citation:

Citation: Baldisseri, Donna; Pelton, J.; Sparks, Steven; Torchia, Dennis. "Complete 1H and 13C assignment of Lys and Leu sidechains of staphylococcal nuclease using HCCH-COSY and HCCH-TOCSY 3D NMR spectroscopy"  FEBS Lett. 281, 33-38 (1991).

Assembly members:

Assembly members:
micrococcal nuclease, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: not available   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts165
15N chemical shifts33
1H chemical shifts341

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1micrococcal nuclease1

Entities:

Entity 1, micrococcal nuclease 156 residues - Formula weight is not available

1   METASPPROTHRVALTYRSERALATHRSER
2   THRLYSLYSLEUHISLYSGLUPROXTHR
3   LEUXLYSALAILEASPGLYXTHRVAL
4   LYSLEUMETTYRLYSGLYXPROMETTHR
5   PHEARGLEULEULEUVALASPTHRPROGLU
6   XLYSXPROLYSLYSGLYVALGLULYS
7   TYRGLYPROXALASERALAPHETHRLYS
8   LYSMETVALGLUASNALALYSLYSILEGLU
9   VALGLUPHEASNLYSGLYGLNXTHRASP
10   LYSTYRGLYARGGLYLEUALAXILETYR
11   ALAASPGLYLYSMETVALASNGLUALALEU
12   VALARGGLNGLYLEUALALYSVALALATYR
13   XTYRLYSPROASNASNTHRHISXX
14   HISLEUARGLYSSERGLUALAGLNALALYS
15   LYSGLULYSLEUASNILEXSERGLUASN
16   ASPALAASPSERGLYGLN

Samples:

sample_one:

sample_condition_set_one: pH: 6.8 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks