BMRB Entry 16874

Title:
The solution structure of rat Lipocalin-type Prostaglandin D Synthase
Deposition date:
2010-04-16
Original release date:
2010-05-17
Authors:
Liu, Jiafu; Lin, Donghai
Citation:

Citation: Liu, Jiafu; Guo, Chenyun; Yao, Yihe; Lin, Donghai. "The solution structure and dynamics of rat Lipocalin-Type Prostaglandin D Synthase"  Biochimie 90, 1637-1646 (2008).
PubMed: 18590794

Assembly members:

Assembly members:
rL-PGDS, polymer, 165 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 22b

Data sets:
Data typeCount
13C chemical shifts599
15N chemical shifts153
1H chemical shifts865

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rat Lipocalin-Type Prostaglandin D Synthase1

Entities:

Entity 1, rat Lipocalin-Type Prostaglandin D Synthase 165 residues - Formula weight is not available

1   GLNGLYHISASPTHRVALGLNPROASNPHE
2   GLNGLNASPLYSPHELEUGLYARGTRPTYR
3   SERALAGLYLEUALASERASNSERSERTRP
4   PHEARGGLULYSLYSGLULEULEUPHEMET
5   CYSGLNTHRVALVALALAPROSERTHRGLU
6   GLYGLYLEUASNLEUTHRSERTHRPHELEU
7   ARGLYSASNGLNCYSGLUTHRLYSVALMET
8   VALLEUGLNPROALAGLYVALPROGLYGLN
9   TYRTHRTYRASNSERPROHISTRPGLYSER
10   PHEHISSERLEUSERVALVALGLUTHRASP
11   TYRASPGLUTYRALAPHELEUPHESERLYS
12   GLYTHRLYSGLYPROGLYGLNASPPHEARG
13   METALATHRLEUTYRSERARGALAGLNLEU
14   LEULYSGLUGLULEULYSGLULYSPHEILE
15   THRPHESERLYSASPGLNGLYLEUTHRGLU
16   GLUASPILEVALPHELEUPROGLNPROASP
17   LYSCYSILEGLNGLU

Samples:

sample_1: rL-PGDS, [U-100% 13C; U-100% 15N], 1.0 mM; H2O 90%; D2O 10%; sodium phosphate 15 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.200 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

GB AAA41839 AAA41840 EDL93575 EDL93576 EDL93577
REF NP_037147
SP P22057
AlphaFold P22057

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks