BMRB Entry 16779

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for LMO2(LIM2)-Ldb1(LID)
Deposition date:
2010-03-18
Original release date:
2010-10-06
Authors:
Wilkinson-White, Lorna; Dastmalchi, Siavoush; Kwan, Ann; Ryan, Daniel; Mackay, Joel; Matthews, Jacqui
Citation:

Citation: Wilkinson-White, Lorna; Dastmalchi, Siavoush; Kwan, Ann; Ryan, Daniel; Mackay, Joel; Matthews, Jacqui. "1H, 15N and 13C assignments of an intramolecular Lmo2-LIM2/Ldb1-LID complex"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
Flic, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Data sets:
Data typeCount
13C chemical shifts435
15N chemical shifts119
1H chemical shifts700

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Lmo2-LIM2/Ldb1-LID1

Entities:

Entity 1, Lmo2-LIM2/Ldb1-LID 123 residues - Formula weight is not available

1   TYRLEUARGLEUPHEGLYGLNASPGLYLEU
2   CYSALASERCYSASPLYSARGILEARGALA
3   TYRGLUMETTHRMETARGVALLYSASPLYS
4   VALTYRHISLEUGLUCYSPHELYSCYSALA
5   ALACYSGLNLYSHISPHESERVALGLYASP
6   ARGTYRLEULEUILEASNSERASPILEVAL
7   CYSGLUGLNASPILETYRGLUTRPTHRLYS
8   ILEASNGLYGLYSERGLYGLYSERGLYGLY
9   SERGLYGLYASPVALMETVALVALGLYGLU
10   PROTHRLEUMETGLYGLYGLUPHEGLYASP
11   GLUASPGLUARGLEUILETHRARGLEUGLU
12   ASNTHRGLNPHEASPALAALAASNGLYILE
13   ASPASPGLU

Samples:

sample_1: Flic, [U-100% 13C; U-100% 15N], 865 uM; DSS 17 uM; D2O 5%; MES 20 mM; DTT 1 mM; H2O 95%; NaCl 150 mM

sample_2: Flic 630 uM; DSS 17 uM; DTT 1 mM; D2O 5%; MES 20 mM; H2O 95%; NaCl 150 mM

sample_3: Flic, [U-100% 13C; U-100% 15N], 865 uM; DSS 17 uM; D2O 100%; MES 20 mM; DTT 1 mM; NaCl 150 mM

sample_conditions_1: pH: 6.5; pressure: ambient atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

TOPSPIN v2.1.b9, Bruker Biospin - collection, processing

TALOS v2009.215.15.29, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB28844 BAE91465 BAG73348
EMBL CAA43430
GB AAA40054 AAF98804 AAH34041 AAH35607 AAH42426
REF NP_001032435 NP_001069820 NP_001135787 NP_001135788 NP_001135807
SP P25791 P25801 Q1LZ94
TPG DAA21843
AlphaFold P25791 Q1LZ94 P25801

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks