BMRB Entry 16776

Title:
C-terminal domain of mesencephalic astrocyte-derived neurotrophic factor (MANF)
Deposition date:
2010-03-12
Original release date:
2022-02-14
Authors:
Hellman, Maarit; Saarma, Mart; Permi, Perttu
Citation:

Citation: Hellman, Maarit; Arumae, Urmas; Yu, Li-ying; Lindholm, Paivi; Peranen, Johan; Saarma, Mart; Permi, Perttu. "Mesencephalic astrocyte-derived neurotrophic factor (MANF) has a unique mechanism to rescue apoptotic neurons"  J. Biol. Chem. 286, 2675-2680 (2011).
PubMed: 21047780

Assembly members:

Assembly members:
C-MANF, polymer, 65 residues, 7476.837 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts227
15N chemical shifts61
1H chemical shifts461

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-MANF1

Entities:

Entity 1, C-MANF 65 residues - 7476.837 Da.

First two residues are cloning artefacts

1   METGLYLYSTYRASPLYSGLNILEASPLEU
2   SERTHRVALASPLEULYSLYSLEUARGVAL
3   LYSGLULEULYSLYSILELEUASPASPTRP
4   GLYGLUTHRCYSLYSGLYCYSALAGLULYS
5   SERASPTYRILEARGLYSILEASNGLULEU
6   METPROLYSTYRALAPROLYSALAALASER
7   ALAARGTHRASPLEU

Samples:

sample_1: C-MANF, [U-99% 13C; U-99% 15N], 1 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C-CT-HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.0.26, P.GUNTERT ET AL. - automated NOE assignment

AMBER v8.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement, structure solution

NMR spectrometers:

  • Varian Unity Inova 600 MHz
  • Varian Unity Inova 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks