BMRB Entry 16720

Title:
Horse prion protein
Deposition date:
2010-02-12
Original release date:
2010-05-18
Authors:
Perez, Daniel; Damberger, Fred; Wuthrich, Kurt
Citation:

Citation: Perez, Daniel; Damberger, Fred; Wuthrich, Kurt. "Horse prion protein NMR structure and comparisons with related variants of the mouse prion protein."  J. Mol. Biol. 400, 121-128 (2010).
PubMed: 20460128

Assembly members:

Assembly members:
mouse prion protein mutant D167S, polymer, 113 residues, 13203.797 Da.

Natural source:

Natural source:   Common Name: Horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSET

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts366
15N chemical shifts131
1H chemical shifts424

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse prion protein mutant D167S1

Entities:

Entity 1, mouse prion protein mutant D167S 113 residues - 13203.797 Da.

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROLEU
3   ILEHISPHEGLYASNASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETTYRARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALSERGLU
6   TYRSERASNGLNLYSASNPHEVALHISASP
7   CYSVALASNILETHRVALLYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPVALLYSILEMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLNLYSGLUTYRGLUALAPHEGLNGLNARG
12   GLYALASER

Samples:

sample_1: sodium acetate, [U-2H], 10 ± 1 mM; H2O 90%; D2O 10%; entity 1.3 ± 1 mM

sample_conditions_1: ionic strength: 10 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

ATHNOS-CANDID, Herrmann and Wuthrich - automatic peak picking and NOE assignment

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAD72795
GB AAD19983 AAD19988 AAD19989 AAD20000 AAN16505
REF NP_001137270 XP_008518008 XP_008518010