BMRB Entry 16640

Name: Solution NMR Structure of 26S protease regulatory subunit 8 from H.sapiens, Northeast Structural Genomics Consortium Target Target HR3102A
Published: 2012-08-03

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PDB ID: 2krk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16640
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR Structure of 26S protease regulatory subunit 8 from H.sapiens, Northeast Structural Genomics Consortium Target Target HR3102A

Deposition date:

2009-12-18

Original release date:

2012-08-03

Authors:

Liu, G.; Janjua, J.; Xiao, R.; Ciccosanti, C.; Shastry, R.; Everett, J.; Nair, R.; Acton, T; Rost, B.; Montelione, G.

Citation:

Citation: Liu, G.; Janjua, J.; Xiao, R.; Ciccosanti, C.; Shastry, R.; Everett, J.; Nair, R.; Acton, T.; Rost, B.; Montelione, G.. "Solution NMR Structure of 26S protease regulatory subunit 8 from H.sapiens, Northeast Structural Genomics Consortium Target HR3102A" To be published ., .-..

Assembly members:

Assembly members:
HR3102A, polymer, 86 residues, 9797.391 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR3102A: MGHHHHHHSHPNEEARLDIL KIHSRKMNLTRGINLRKIAE LMPGASGAEVKGVCTEAGMY ALRERRVHVTQEDFEMAVAK VMQKDS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	259
15N chemical shifts	84
1H chemical shifts	564

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HR3102A	1

Entities:

Entity 1, HR3102A 86 residues - 9797.391 Da.

1

MET

GLY

HIS

SER

HIS

2

PRO

ASN

GLU

ALA

ARG

LEU

ASP

ILE

LEU

3

LYS

ILE

HIS

SER

ARG

LYS

MET

ASN

LEU

THR

4

ARG

GLY

ILE

ASN

LEU

ARG

LYS

ILE

ALA

GLU

5

LEU

MET

PRO

GLY

ALA

SER

GLY

ALA

GLU

VAL

6

LYS

GLY

VAL

CYS

THR

GLU

ALA

GLY

MET

TYR

7

ALA

LEU

ARG

GLU

ARG

VAL

HIS

VAL

THR

8

GLN

GLU

ASP

PHE

GLU

MET

ALA

VAL

ALA

LYS

9

VAL

MET

GLN

LYS

ASP

SER

Samples:

sample_1: HR3102A, [U-100% 13C; U-100% 15N], 0.82 mM; H2O 95%; D2O 5%

sample_2: HR3102A, [U-10% 13C; U-100% 15N], 0.52 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 1; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-13C arom NOESY	sample_1	isotropic	sample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
GFT HABCABcoNHN	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinemen,structure solution,geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement,geometry optimization,structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis,refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - data analysis,chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis,peak picking,chemical shift assignment

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

NMR spectrometers:

Bruker Avance 800 MHz
Varian INOVA 600 MHz

PDB	2KRK
DBJ	BAA07919 BAA11938 BAA22933 BAA22935 BAB26990
EMBL	CAA57512 CAA61863 CAA61864 CAA90961 CAG12637
GB	AAB88187 AAC19266 AAC41735 AAC46996 AAC48284
PRF	2106382A 2111282A
REF	NP_001003740 NP_001081635 NP_001085482 NP_001127417 NP_001134720
SP	O18413 P46470 P54814 P62194 P62195
TPG	DAA18341
AlphaFold	P62194 P54814 O18413 P46470 P62195