BMRB Entry 16521

Title:
NMR solution structure of CV_2116 from Chromobacterium violaceum.Northeast Structural Genomics Consortium Target CvT4(1-82)
Deposition date:
2009-09-24
Original release date:
2014-03-04
Authors:
Yang, Yunhuang; Ramelot, Theresa; Cort, John; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl; Montelio, Gaetano; Kennedy, Michael
Citation:

Citation: Yang, Yunhuang; Ramelot, Theresa; Cort, John; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl; Montelione, Gaetano; Kennedy, Michael. "NMR solution structure of CV_2116 from Chromobacterium violaceum. Northeast Structural Genomics Consortium Target CvT4(1-82)"  .

Assembly members:

Assembly members:
CV_2116, polymer, 82 residues, 9399.5 Da.

Natural source:

Natural source:   Common Name: Chromobacterium violaceum   Taxonomy ID: 536   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Chromobacterium violaceum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p15Tv lic

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts371
15N chemical shifts87
1H chemical shifts589

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 82 residues - 9399.5 Da.

1   METASNVALALAHISTYRARGGLYTYRGLU
2   ILEGLUPROGLYHISGLNTYRARGASPASP
3   ILEARGLYSTYRVALPROTYRALALEUILE
4   ARGLYSVALGLYVALPROASPARGTHRPRO
5   ILEPROTHRTHRTYRPROGLUPHETYRASP
6   LEUGLUALAASPALAGLUARGVALSERILE
7   ALACYSALALYSILEILEILEASPSERHIS
8   LEUASPARGHISASPGLNGLYLEUALAASP
9   LEUGLY

Samples:

NC_sample: CV_2116, [U-100% 13C; U-100% 15N], 0.8 ± 0.1 mM; TRIS 10 ± 0.5 mM; sodium chloride 300 ± 15 mM; zinc sulfate 10 ± 0.5 uM; DTT 10 ± 0.5 mM; benzamidine 1 ± 0.05 mM; inhibitor cocktail 1 1/1 v/v

NC7_sample: CV_2116 0.8 ± 0.1 mM; TRIS 10 ± 0.5 mM; sodium chloride 300 ± 15 mM; zinc sulfate 10 ± 0.5 uM; DTT 10 ± 0.5 mM; benzamidine 1 ± 0.05 mM; inhibitor cocktail 1 1/1 v/v

NC_sample_in_D2O: CV_2116, [U-100% 13C; U-100% 15N], 0.8 ± 0.1 mM; TRIS 10 ± 0.5 mM; sodium chloride 300 ± 15 mM; zinc sulfate 10 ± 0.05 uM; DTT 10 ± 0.5 mM; benzamidine 1 ± 0.05 mM; inhibitor cocktail 1 1/1 v/v

sample_conditions_1: ionic strength: 0.2 M; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNC_sampleisotropicsample_conditions_1
2D 1H-13C HSQCNC_sampleisotropicsample_conditions_1
2D 1H-13C HSQCNC7_sampleisotropicsample_conditions_1
3D 1H-15N NOESYNC_sampleisotropicsample_conditions_1
3D 1H-13C NOESYNC_sampleisotropicsample_conditions_1
3D HNCONC_sampleisotropicsample_conditions_1
3D HNCANC_sampleisotropicsample_conditions_1
3D HNCACBNC_sampleisotropicsample_conditions_1
3D CBCA(CO)NHNC_sampleisotropicsample_conditions_1
3D HN(CO)CANC_sampleisotropicsample_conditions_1
3D HBHA(CO)NHNC_sampleisotropicsample_conditions_1
3D C(CO)NHNC_sampleisotropicsample_conditions_1
3D HCCH-TOCSYNC_sampleisotropicsample_conditions_1
3D HCCH-COSYNC_sampleisotropicsample_conditions_1
3D (H)CCH-TOCSYNC_sample_in_D2Oisotropicsample_conditions_1
4D CC NOESYNC_sample_in_D2Oisotropicsample_conditions_1
2D His-HMQCNC_sampleisotropicsample_conditions_1

Software:

NMRPipe v2008, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR v6.1C, Varian - collection

TOPSPIN v2.1.3, Bruker Biospin - collection

AutoStruct v2.2.1, Huang, Tejero, Powers and Montelione - data analysis

X-PLOR NIH v2.20, Schwieters, Kuszewski, Tjandra and Clore - structure solution

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

SPARKY v3.113, Goddard - data analysis

PSVS v1.4, Bhattacharya and Montelione - structure solution

AutoAssign v2.30, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

PDBStat v5.1, (PdbStat)-Roberto Tejero and Gaetano T. Montelione - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

SWS Q7NW74
PDB
GB AAQ59789
REF WP_011135664

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks