BMRB Entry 16446

Name: Chemical shift assignments for Ncs1p
Published: 2009-11-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16446

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments for Ncs1p

Deposition date:

2009-08-10

Original release date:

2009-11-19

Authors:

Ames, James

Citation:

Citation: Lim, Sunghyuk; Ames, James. "(1)H, (15)N, and (13)C chemical shift assignments of neuronal calcium sensor-1 homolog from fission yeast." Biomol NMR Assign 3, 269-271 (2009).
PubMed: 19851889

Assembly members:

Assembly members:
Ncs1p, polymer, 190 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Fission Yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ncs1p: MGKSQSKLSQDQLQDLVRST RFDKKELQQWYKGFFKDCPS GHLNKSEFQKIYKQFFPFGD PSAFAEYVFNVFDADKNGYI DFKEFICALSVTSRGELNDK LIWAFQLYDLDNNGLISYDE MLRIVDAIYKMVGSMVKLPE DEDTPEKRVNKIFNMMDKNK DGQLTLEEFCEGSKRDPTIV SALSLYDGLV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	600
15N chemical shifts	184
1H chemical shifts	1034

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 190 residues - Formula weight is not available

1	MET	GLY	LYS	SER	GLN	SER	LYS	LEU	SER	GLN
2	ASP	GLN	LEU	GLN	ASP	LEU	VAL	ARG	SER	THR
3	ARG	PHE	ASP	LYS	LYS	GLU	LEU	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	PHE	LYS	ASP	CYS	PRO	SER
5	GLY	HIS	LEU	ASN	LYS	SER	GLU	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	SER	ALA	PHE	ALA	GLU	TYR	VAL	PHE	ASN
8	VAL	PHE	ASP	ALA	ASP	LYS	ASN	GLY	TYR	ILE
9	ASP	PHE	LYS	GLU	PHE	ILE	CYS	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	GLU	LEU	ASN	ASP	LYS
11	LEU	ILE	TRP	ALA	PHE	GLN	LEU	TYR	ASP	LEU
12	ASP	ASN	ASN	GLY	LEU	ILE	SER	TYR	ASP	GLU
13	MET	LEU	ARG	ILE	VAL	ASP	ALA	ILE	TYR	LYS
14	MET	VAL	GLY	SER	MET	VAL	LYS	LEU	PRO	GLU
15	ASP	GLU	ASP	THR	PRO	GLU	LYS	ARG	VAL	ASN
16	LYS	ILE	PHE	ASN	MET	MET	ASP	LYS	ASN	LYS
17	ASP	GLY	GLN	LEU	THR	LEU	GLU	GLU	PHE	CYS
18	GLU	GLY	SER	LYS	ARG	ASP	PRO	THR	ILE	VAL
19	SER	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL

Samples:

sample_1: Ncs1p, [U-95% 13C; U-95% 15N], 1.0 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2L2E
EMBL	CAA90589
GB	AAP48992
REF	NP_592879
SP	Q09711
AlphaFold	Q09711

1	MET	GLY	LYS	SER	GLN	SER	LYS	LEU	SER	GLN
2	ASP	GLN	LEU	GLN	ASP	LEU	VAL	ARG	SER	THR
3	ARG	PHE	ASP	LYS	LYS	GLU	LEU	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	PHE	LYS	ASP	CYS	PRO	SER
5	GLY	HIS	LEU	ASN	LYS	SER	GLU	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	SER	ALA	PHE	ALA	GLU	TYR	VAL	PHE	ASN
8	VAL	PHE	ASP	ALA	ASP	LYS	ASN	GLY	TYR	ILE
9	ASP	PHE	LYS	GLU	PHE	ILE	CYS	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	GLU	LEU	ASN	ASP	LYS
11	LEU	ILE	TRP	ALA	PHE	GLN	LEU	TYR	ASP	LEU
12	ASP	ASN	ASN	GLY	LEU	ILE	SER	TYR	ASP	GLU
13	MET	LEU	ARG	ILE	VAL	ASP	ALA	ILE	TYR	LYS
14	MET	VAL	GLY	SER	MET	VAL	LYS	LEU	PRO	GLU
15	ASP	GLU	ASP	THR	PRO	GLU	LYS	ARG	VAL	ASN
16	LYS	ILE	PHE	ASN	MET	MET	ASP	LYS	ASN	LYS
17	ASP	GLY	GLN	LEU	THR	LEU	GLU	GLU	PHE	CYS
18	GLU	GLY	SER	LYS	ARG	ASP	PRO	THR	ILE	VAL
19	SER	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL

1	MET	GLY	LYS	SER	GLN	SER	LYS	LEU	SER	GLN
2	ASP	GLN	LEU	GLN	ASP	LEU	VAL	ARG	SER	THR
3	ARG	PHE	ASP	LYS	LYS	GLU	LEU	GLN	GLN	TRP
4	TYR	LYS	GLY	PHE	PHE	LYS	ASP	CYS	PRO	SER
5	GLY	HIS	LEU	ASN	LYS	SER	GLU	PHE	GLN	LYS
6	ILE	TYR	LYS	GLN	PHE	PHE	PRO	PHE	GLY	ASP
7	PRO	SER	ALA	PHE	ALA	GLU	TYR	VAL	PHE	ASN
8	VAL	PHE	ASP	ALA	ASP	LYS	ASN	GLY	TYR	ILE
9	ASP	PHE	LYS	GLU	PHE	ILE	CYS	ALA	LEU	SER
10	VAL	THR	SER	ARG	GLY	GLU	LEU	ASN	ASP	LYS
11	LEU	ILE	TRP	ALA	PHE	GLN	LEU	TYR	ASP	LEU
12	ASP	ASN	ASN	GLY	LEU	ILE	SER	TYR	ASP	GLU
13	MET	LEU	ARG	ILE	VAL	ASP	ALA	ILE	TYR	LYS
14	MET	VAL	GLY	SER	MET	VAL	LYS	LEU	PRO	GLU
15	ASP	GLU	ASP	THR	PRO	GLU	LYS	ARG	VAL	ASN
16	LYS	ILE	PHE	ASN	MET	MET	ASP	LYS	ASN	LYS
17	ASP	GLY	GLN	LEU	THR	LEU	GLU	GLU	PHE	CYS
18	GLU	GLY	SER	LYS	ARG	ASP	PRO	THR	ILE	VAL
19	SER	ALA	LEU	SER	LEU	TYR	ASP	GLY	LEU	VAL