BMRB Entry 16405

Name: Solution Structure of the Tandem UIM Domain of Ataxin-3 Complexed with Ubiquitin
Published: 2012-08-03

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PDB ID: 2klz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16405
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Tandem UIM Domain of Ataxin-3 Complexed with Ubiquitin

Deposition date:

2009-07-12

Original release date:

2012-08-03

Authors:

Zhou, Chenjie; Song, Aixin; Lin, Donghai; Hu, Hongyu

Citation:

Citation: Song, Ai-Xin; Zhou, Chen-Jie; Peng, Yu; Gao, Xue-Chao; Zhou, Zi-Ren; Fu, Qing-Shan; Hong, Jing; Lin, Dong-Hai; Hu, Hong-Yu. "Structural Transformation of the Tandem Ubiquitin-Interacting Motifs in Ataxin-3 and Their Cooperative Interactions with Ubiquitin Chains" PLoS ONE 5, e13202-e13202 (2010).
PubMed: 20949063

Assembly members:

Assembly members:
Ataxin-3/Ubiquitin complex, polymer, 46 residues, 5268.689 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGBTNH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ataxin-3/Ubiquitin complex: GSLDEDEEDLQRALALSRQE IDMEDEEADLRRAIQLSMQG SSRNLE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	133
15N chemical shifts	41
1H chemical shifts	241

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ataxin-3/Ubiquitin complex	1

Entities:

Entity 1, Ataxin-3/Ubiquitin complex 46 residues - 5268.689 Da.

1

GLY

SER

LEU

ASP

GLU

ASP

GLU

ASP

LEU

2

GLN

ARG

ALA

LEU

ALA

LEU

SER

ARG

GLN

GLU

3

ILE

ASP

MET

GLU

ASP

GLU

ALA

ASP

LEU

4

ARG

ALA

ILE

GLN

LEU

SER

MET

GLN

GLY

5

SER

ARG

ASN

LEU

GLU

Samples:

sample_1: entity, [U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02%; ubiquitin 4 mM

sample_2: entity, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02%; ubiquitin 4 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

Varian INOVA 600 MHz

PDB	2KLZ
GB	ADD00635 EHB02478 ELW55804
REF	XP_004584394 XP_004681665 XP_004681676 XP_004837073 XP_009004709