BMRB Entry 16356

Title:
Structure of a two-G-tetrad intramolecular G-quadruplex formed by a variant human telomeric sequence in K+ solution
Deposition date:
2009-06-16
Original release date:
2009-12-11
Authors:
Zhang, Zhengjiang; Dai, Jixun; Yang, Danzhou
Citation:

Citation: Zhang, Zhenjiang; Dai, Jixun; Veliath, Elizabeth; Jones, Roger; Yang, Danzhou. "Structure of a two-G-tetrad intramolecular G-quadruplex formed by a variant human telomeric sequence in K+ solution: insights into the interconversion of human telomeric G-quadruplex structures."  Nucleic Acids Res. 38, 1009-1021 (2010).
PubMed: 19946019

Assembly members:

Assembly members:
DNA(5'-D(*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*(INO)P*GP*GP*TP*TP*AP*GP*GP*GP*T)-3'), polymer, 23 residues, 7095.636 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA(5'-D(*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*(INO)P*GP*GP*TP*TP*AP*GP*GP*GP*T)-3'): AGGGTTAGGGTTAXGGTTAG GGT

Data sets:
Data typeCount
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA(5'-D(*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*(INO)P*GP*GP*TP*TP*AP*GP*GP*GP*T)-3')1

Entities:

Entity 1, DNA(5'-D(*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*(INO)P*GP*GP*TP*TP*AP*GP*GP*GP*T)-3') 23 residues - 7095.636 Da.

1   DADGDGDGDTDTDADGDGDG
2   DTDTDAINODGDGDTDTDADG
3   DGDGDT

Samples:

sample_1: entity 1 mM; potassium chloride 60 mM; potassium phosphate 30 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 90 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz