BMRB Entry 16236

Title:
Bruno RRM3+
Deposition date:
2009-04-01
Original release date:
2009-11-23
Authors:
Lyon, Angeline; Reveal, Brad; Macdonald, Paul; Hoffman, David
Citation:

Citation: Lyon, Angeline; Reveal, Brad; Macdonald, Paul; Hoffman, David. "Bruno protein contains an expanded RNA recognition motif"  Biochemistry 48, 12202-12212 (2009).
PubMed: 19919093

Assembly members:

Assembly members:
Bruno_RRM3+, polymer, 118 residues, 12296.124 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
13C chemical shifts434
15N chemical shifts117
1H chemical shifts782

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bruno_RRM3+1

Entities:

Entity 1, Bruno_RRM3+ 118 residues - 12296.124 Da.

1   ALAALAGLYLEUPROGLNPHEGLYSERALA
2   SERALALEUSERTHRSERPROLEUALASER
3   VALALALEUSERALAALAALAALAALAALA
4   ALAGLYLYSGLNILEGLUGLYPROGLUGLY
5   CYSASNLEUPHEILETYRHISLEUPROGLN
6   GLUPHETHRASPTHRASPLEUALASERTHR
7   PHELEUPROPHEGLYASNVALILESERALA
8   LYSVALPHEILEASPLYSGLNTHRSERLEU
9   SERLYSCYSPHEGLYPHEVALSERPHEASP
10   ASNPROASPSERALAGLNVALALAILELYS
11   ALAMETASNGLYPHEGLNVALGLYTHRLYS
12   ARGLEULYSVALGLNLEULYSLYS

Samples:

Bruno_RRM3+_1: 1,0 mM Bruno RRM3+, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 1 mM; sodium chloride300 – 400 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 260 mM; pH: 6; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYBruno_RRM3+_1isotropicsample_conditions_1
3D 1H-15N NOESYBruno_RRM3+_1isotropicsample_conditions_1
2D 1H-1H NOESYBruno_RRM3+_1isotropicsample_conditions_1

Software:

CNS v1.1., Brunger, Adams, Clore, Gros, Nilges and Read - simulated annealing

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

PDB
GB AAL49272 AAM50707 AAN10813 ACL86931 AET11743
REF NP_723740 XP_002015057

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks