BMRB Entry 16124

Title:
Solution structure of RppH from Escherichia coli
Deposition date:
2009-01-18
Original release date:
2009-04-14
Authors:
Bi, Yunchen; Li, Hongwei; Jin, Changwen
Citation:

Citation: Bi, Yunchen; Li, Hongwei; Fan, Shoujin; Xia, Bin; Jin, Changwen. "1H, 13C and 15N resonance assignments of RNA pyrophosphohydrolase RppH from Escherichia coli"  Biomol. NMR Assignments 3, 149-151 (2009).
PubMed: 19636968

Assembly members:

Assembly members:
RppH, polymer, 164 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21

Data sets:
Data typeCount
13C chemical shifts717
15N chemical shifts187
1H chemical shifts1107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RppH1

Entities:

Entity 1, RppH 164 residues - Formula weight is not available

1   METILEASPASPASPGLYTYRARGPROASN
2   VALGLYILEVALILECYSASNARGGLNGLY
3   GLNVALMETTRPALAARGARGPHEGLYGLN
4   HISSERTRPGLNPHEPROGLNGLYGLYILE
5   ASNPROGLYGLUSERALAGLUGLNALAMET
6   TYRARGGLULEUPHEGLUGLUVALGLYLEU
7   SERARGLYSASPVALARGILELEUALASER
8   THRARGASNTRPLEUARGTYRLYSLEUPRO
9   LYSARGLEUVALARGTRPASPTHRLYSPRO
10   VALCYSILEGLYGLNLYSGLNLYSTRPPHE
11   LEULEUGLNLEUVALSERGLYASPALAGLU
12   ILEASNMETGLNTHRSERSERTHRPROGLU
13   PHEASPGLYTRPARGTRPVALSERTYRTRP
14   TYRPROVALARGGLNVALVALSERPHELYS
15   ARGASPVALTYRARGARGVALMETLYSGLU
16   PHEALASERVALVALMETSERLEUGLNGLU
17   ASNTHRPROLYS

Samples:

sample_1: sodium acetate 50 mM; sodium chloride 20 mM; DTT 40 mM; arginine 10 mM; glutamic 10 mM; RppH protein, [U-13C; U-15N], 0.8 mM

sample_conditions_1: ionic strength: 90 mM; pH: 5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

xwinnmr, Bruker Biospin - collection

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB37110 BAE76899 BAG78611 BAI27093 BAI32136
EMBL CAD02829 CAP77286 CAQ33156 CAQ90258 CAQ99756
GB AAB40477 AAC75869 AAG57941 AAL21880 AAN44326
PIR AE0866
REF NP_311714 NP_417307 NP_457398 NP_461921 NP_708619
SP A7ZQT6 A8A3W3 A9MS78 A9N2M1 B1IU14
AlphaFold A9N2M1 B1IU14 A9MS78 A8A3W3 A7ZQT6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks