BMRB Entry 16118

Title:
Solution structure of PFE0790c, a putative bolA-like protein from the protozoan parasite Plasmodium falciparum: A Structural Genomics Center for Infectious Disease (SSGCID) community request.
Deposition date:
2009-01-12
Original release date:
2009-03-05
Authors:
Buchko, Garry; Yee, Adelinda; Semesi, Anthony; Hui, Raymond; Arrowsmith, Cheryl
Citation:

Citation: Buchko, Garry; Yee, Adelinda; Semesi, Anthony; Arrowsmith, Cheryl; Hui, Raymond. "Structural characterization of PFE0790c, a putative bolA-like protein from Plasmodium falciparum, the protozoan parasite responsible for malaria."  .

Assembly members:

Assembly members:
PFE0790c, polymer, 108 residues, 12328.188 Da.

Natural source:

Natural source:   Common Name: Plasmodium falciparum   Taxonomy ID: 36329   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: lic

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts95
1H chemical shifts607

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PFE0790c1

Entities:

Entity 1, PFE0790c 108 residues - 12328.188 Da.

N-terminal residues 1-22 and C-terminal residues 107-108 are not native. The first residue in the native sequence of PFE0790c is M23.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETCYSILEGLNLYSVALILEGLU
4   ASPLYSLEUSERSERALALEULYSPROTHR
5   PHELEUGLULEUVALASPLYSSERCYSGLY
6   CYSGLYTHRSERPHEASPALAVALILEVAL
7   SERASNASNPHEGLUASPLYSLYSLEULEU
8   ASPARGHISARGLEUVALASNTHRILELEU
9   LYSGLUGLULEUGLNASNILEHISALAPHE
10   SERMETLYSCYSHISTHRPROLEUGLUTYR
11   ASPLYSLEULYSSERLYSGLYSER

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 1.5 ± 0.5 mM; sodium phosphate 10 ± 1 mM; sodium chloride 200 ± 5 mM; DTT 10 ± 1 mM; sodium azide 0.01 ± 0.002 %; benzamidine 1 ± 0.01 mM; H2O 93%; D2O 7%

sample_2: entity, [U-7% 13C; U-99% 15N], 1.5 ± 0.5 mM; sodium phosphate 10 ± 1 mM; sodium chloride 200 ± 5 mM; DTT 10 ± 1 mM; sodium azide 0.01 ± 0.002 %; benzamidine 1 ± 0.01 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY v3.110, Goddard - data analysis, peak picking

FELIX v2007, Accelrys Software Inc. - processing

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

PSVS v1.3, Bhattacharya and Montelione - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CAD51522 CDO63036
GB KOB58477
REF XP_001351715 XP_012761671

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks