BMRB Entry 15828

Title:
NMR Structure for PF0385
Deposition date:
2008-06-26
Original release date:
2008-08-20
Authors:
Prestegard, James; Tian, Fang; Baucom, Clay; Adams, Michael; Sugar, Frank
Citation:

Citation: Prestegard, James; Tian, Fang; Baucom, Clay; Adams, Michael; Sugar, Frank. "NMR structure of PF0385"  .

Assembly members:

Assembly members:
PF0385, polymer, 88 residues, 10472.290 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet27

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts247
15N chemical shifts85
1H chemical shifts467

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PF03851

Entities:

Entity 1, PF0385 88 residues - 10472.290 Da.

1   METALALYSCYSPROILECYSGLYSERPRO
2   LEULYSTRPGLUGLULEUILEGLUGLUMET
3   LEUILEILEGLUASNPHEGLUGLUILEVAL
4   LYSASPARGGLUARGPHELEUALAGLNVAL
5   GLUGLUPHEVALPHELYSCYSPROVALCYS
6   GLYGLUGLUPHETYRGLYLYSTHRLEUPRO
7   ARGARGGLUALAGLULYSVALPHEGLULEU
8   LEUASNASPPHELYSGLYGLYILEASPTRP
9   GLUASNLYSARGVALLYSLEULYS

Samples:

sample_1: PF0385, [U-100% 13C; U-100% 15N], 1.2 mM; Tris-Mops 20 mM; DTT 5 mM; KCl 50 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: PF0385, [U-100% 13C; U-100% 15N], 0.8 mM; Tris-Mops 20 mM; DTT 5 mM; KCl 50 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQC-IPAPsample_2anisotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAL80509 AFN03141
REF WP_011011499

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks