BMRB Entry 15759

Title:
1H, 13C and 15N backbone chemical shift assignments of the 3FNIII module from human fibronectin
Deposition date:
2008-05-06
Original release date:
2009-04-15
Authors:
Vakonakis, Ioannis; Campbell, Iain
Citation:

Citation: Vakonakis, Ioannis; Staunton, David; Ellis, Ian; Sarkies, Peter; Flanagan, Aleksandra; Schor, Ana; Schor, Seth; Campbell, Iain. "Motogenic sites in human fibronectin are masked by long range interactions"  J. Biol. Chem. 284, 15668-15675 (2009).
PubMed: 19366708

Assembly members:

Assembly members:
3F3, polymer, 96 residues, 10256.2 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-2

Data sets:
Data typeCount
13C chemical shifts178
15N chemical shifts80
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1

Entities:

Entity 1, protein 96 residues - 10256.2 Da.

1   GLYPROLEUGLYSERALAPROASPALAPRO
2   PROASPPROTHRVALASPGLNVALASPASP
3   THRSERILEVALVALARGTRPSERARGPRO
4   GLNALAPROILETHRGLYTYRARGILEVAL
5   TYRSERPROSERVALGLUGLYSERSERTHR
6   GLULEUASNLEUPROGLUTHRALAASNSER
7   VALTHRLEUSERASPLEUGLNPROGLYVAL
8   GLNTYRASNILETHRILETYRALAVALGLU
9   GLUASNGLNGLUSERTHRPROVALVALILE
10   GLNGLNGLUTHRTHRGLY

Samples:

sample_1: 3F3 0.7 mM; D2O 5%; DSS 0.1 mM; sodium chloride 20 mM; sodium phosphate 20 mM; sodium azide 0.01%

sample_conditions_1: ionic strength: 115 mM; pH: 6; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP v4.3.7, Garrett - data analysis

TOPSPIN v1.3, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 25564
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks