BMRB Entry 15743

Title:
Sequence specific 1H, 13C and 15N resonance assignment of Hahellin from Hahella chejuensis, a putative member of the bg-crystallin superfamily
Deposition date:
2008-04-25
Original release date:
2008-09-12
Authors:
Srivastava, Atul; Chary, Kandala; Sharma, Yogendra
Citation:

Citation: Srivastava, Atul; Sharma, Yogendra; Chary, Kandala. "Sequence specific 1H, 13C, and 15N resonance assignments of Hahellin from Hahella chejuensis, a putative member of the betagamma-crystallin superfamily"  Biomol. NMR Assignments 2, 151-153 (2008).
PubMed: 19636892

Assembly members:

Assembly members:
hahellin, polymer, 92 residues, Formula weight is not available
CA, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Hahella chejuensis   Taxonomy ID: 158327   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Hahella chejuensis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts341
15N chemical shifts92
1H chemical shifts558

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Subunit 11
2calcium ion2

Entities:

Entity 1, Subunit 1 92 residues - Formula weight is not available

1   METGLYGLULYSTHRVALLYSLEUTYRGLU
2   ASPTHRHISPHELYSGLYTYRSERVALGLU
3   LEUPROVALGLYASPTYRASNLEUSERSER
4   LEUILESERARGGLYALALEUASNASPASP
5   LEUSERSERALAARGVALPROSERGLYLEU
6   ARGLEUGLUVALPHEGLNHISASNASNPHE
7   LYSGLYVALARGASPPHETYRTHRSERASP
8   ALAALAGLULEUSERARGASPASNASPALA
9   SERSERVALARGVALSERLYSMETGLUTHR
10   THRASN

Entity 2, calcium ion - Formula weight is not available

1   CA

Samples:

sample_1: sodium chloride 0.8 mM; sodium chloride 300 mM; Tris.Cl 10 mM; calcium chloride 20 mM

sample_conditions_1: pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - processing

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16777 18446
PDB
GB ABC29838
REF WP_011396907

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks