BMRB Entry 15595

Title:
A novel solution NMR structure of protein yst0336 from Saccharomyces/Northest Structural Genomics Consortium Target YT51/Ontario Center for Structural Proteomics
Deposition date:
2007-12-14
Original release date:
2008-01-15
Authors:
Wu, Bin; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Gutmanas, Aleksandras; Semest, Anthony; Arrowsmith, Cheryl
Citation:

Citation: Wu, Adelinda; Yee, Adelinda; Fares, Christophe; Lemak, Alexander; Gutmanas, Aleksandras; Semest, Anthony; Arrowsmith, Cheryl. "A Novel solution NMR structure of protein yst0336 from Saccharomyces cerevisiae/North Structural Genomics Consortium Target YT51/Ontario Center for Structural Proteomics Target yst0336"  .

Assembly members:

Assembly members:
yst0336, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts627
15N chemical shifts155
1H chemical shifts1038

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yst03361

Entities:

Entity 1, yst0336 146 residues - Formula weight is not available

1   METSERTHRPHEASNALAGLUTHRALAASP
2   ASNLEUGLUASPILEGLULYSGLNPHEALA
3   VALVALALAVALGLUGLNALAGLUTHRTYR
4   TRPLYSLEULEUTHRSERVALPROGLYSER
5   LYSLEUARGLEUTHRLYSPHEASPASPGLU
6   ILETYRGLUASNPHEMETGLUARGPHEPRO
7   GLUTYRLYSASPVALGLUARGVALLYSLYS
8   PHETHRGLUGLUGLULEULYSTHRLYSGLU
9   ALALYSGLUARGTRPARGLYSPHEPHETHR
10   ILEPHEGLULYSLYSILEGLUASPTYRASN
11   PHEGLYTHRLEULEUARGTHRASPALASER
12   ALAGLUTYRGLYGLNPHETHRTHRCYSPHE
13   VALVALARGLEUGLNPHETYRALAPHEGLU
14   ILEALAARGASNLYSHISGLYLEUASNASP
15   TRPILEVALGLYGLNLYS

Samples:

sample_1: unknown function protein yst0336 from Saccharomyces cerevisiae, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; sodium azide 0.01%; benzamidine 10 mM

sample_2: unknown function protein yst0336 from Saccharomyces cerevisiae, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; sodium azide 0.01%; benzamidine 10 mM

sample_3: unknown function protein yst0336 from Saccharomyces cerevisiae, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; sodium azide 0.01%; benzamidine 10 mM

sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.95, Goddard - data analysis, peak picking

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.0, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

AutoStruct, Huang, Tejero, Powers and Montelione - NMR structure quality assessment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ GAA26753
EMBL CAA97940 CAY86735
GB AAT92581 AHY77958 AJP41926 AJU23370 AJU24058
REF NP_015099
SP Q08971
TPG DAA11211
AlphaFold Q08971

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks