BMRB Entry 15582

Title:
NS3(10-24)
Deposition date:
2007-12-05
Original release date:
2008-09-05
Authors:
Montserret, Roland; Penin, Francois
Citation:

Citation: Brass, Volker; Berke, Jan Martin; Montserret, Roland; Blum, Hubert; Penin, Francois; Moradpour, Darius. "Structural determinants for membrane association and dynamic organization of the hepatitis C virus NS3-4A complex"  Proc. Natl. Acad. Sci. U. S. A. 105, 14545-14550 (2008).
PubMed: 18799730

Assembly members:

Assembly members:
NS3(10-24), polymer, 15 residues, 1562.886 Da.

Natural source:

Natural source:   Common Name: Hepatitis C Virus   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepatitis C virus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NS3(10-24): TRGLLGCIITSLTGR

Data sets:
Data typeCount
13C chemical shifts44
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 15 residues - 1562.886 Da.

1   THRARGGLYLEULEUGLYCYSILEILETHR
2   SERLEUTHRGLYARG

Samples:

sample_1: NS3(10-24) 1.5 ± 0.1 mM; Sodium Dodecyl Sulfate, [U-100% 2H], 50 mM

sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

DBJ BAA03905 BAB18806 BAB18813 BAC77767 BAH08638
EMBL CAB46911 CAB46913 CAB46915 CAB46917
GB AAA45677 AAF61205 AAF65947 AAF65950 AAV39275