BMRB Entry 15519

Title:
Backbone chemical shift assigment of chicken osteopontin
Deposition date:
2007-10-11
Original release date:
2007-11-15
Authors:
Schedlbauer, Andreas; Kontaxis, Georg; Konrat, Robert; Bister, Klaus
Citation:

Citation: Schedlbauer, Andreas; Ozdowy, Przemyslaw; Kontaxis, Georg; Hartl, Markus; Bister, Klaus; Konrat, Robert. "Backbone assignment of osteopontin, a cytokine and cell attachment protein implicated in tumorigenesis."  Biomol. NMR Assignments 2, 29-31 (2008).
PubMed: 19636917

Assembly members:

Assembly members:
opn, polymer, 220 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pCAT-OPN

Data sets:
Data typeCount
13C chemical shifts436
15N chemical shifts183
1H chemical shifts181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1opn1

Entities:

Entity 1, opn 220 residues - Formula weight is not available

1   METHISGLNASPHISVALASPSERGLNSER
2   GLNGLUHISLEUGLNGLNTHRGLNASNASP
3   LEUALASERLEUGLNGLNTHRHISTYRSER
4   SERGLUGLUASNALAASPVALPROGLUGLN
5   PROASPPHEPROASPVALPROSERLYSSER
6   GLNGLUTHRVALASPASPASPASPASPASP
7   ASPASNASPSERASNASPTHRASPGLUSER
8   ASPGLUVALPHETHRASPPHEPROTHRGLU
9   ALAPROVALALAPROPHEASNARGGLYASP
10   ASNALAGLYARGGLYASPSERVALALATYR
11   GLYPHEARGALALYSALAHISVALVALLYS
12   ALASERLYSILEARGLYSALAALAARGLYS
13   LEUILEGLUASPASPALATHRTHRGLUASP
14   GLYASPSERGLNPROALAGLYLEUTRPTRP
15   PROLYSGLUSERARGGLUGLNASNSERARG
16   GLULEUPROGLNHISGLNSERVALGLUASN
17   ASPSERARGPROLYSPHEASPSERARGGLU
18   VALASPGLYGLYASPSERLYSALASERALA
19   GLYVALASPSERARGGLUSERGLNGLYSER
20   VALPROALAVALASPALASERASNGLNTHR
21   LEUGLUSERALAGLUASPALAGLUASPARG
22   HISSERILEGLUASNASNGLUVALTHRARG

Samples:

sample_1: opn, [U-99% 13C; U-99% 15N], 1.4 mM; NaPhosphate 100 mM; NaCl 150 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CA)COsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(C)Nsample_1isotropicsample_conditions_1

Software:

SPARKY v3.110, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

GB AAF63330

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks