BMRB Entry 15498

Title:
Backbone and side-chain 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH7
Deposition date:
2007-09-27
Original release date:
2008-01-17
Authors:
Serniwka, Stephanie; Shaw, Gary
Citation:

Citation: Serniwka, Stephanie; Shaw, Gary. "1H, 13C and 15N resonance assignments for the human E2 conjugating enzyme, UbcH7"  Biomol. NMR Assignments 2, 21-23 (2008).
PubMed: 19636915

Assembly members:

Assembly members:
UbcH7, polymer, 153 residues, 18684 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
13C chemical shifts602
15N chemical shifts150
1H chemical shifts736

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UbcH71

Entities:

Entity 1, UbcH7 153 residues - 18684 Da.

1   ALAALASERARGARGLEUMETLYSGLULEU
2   GLUGLUILEARGLYSSERGLYMETLYSASN
3   PHEARGASNILEGLNVALASPGLUALAASN
4   LEULEUTHRTRPGLNGLYLEUILEVALPRO
5   ASPASNPROPROTYRASPLYSGLYALAPHE
6   ARGILEGLUILEASNPHEPROALAGLUTYR
7   PROPHELYSPROPROLYSILETHRPHELYS
8   THRLYSILETYRHISPROASNILEASPGLU
9   LYSGLYGLNVALCYSLEUPROVALILESER
10   ALAGLUASNTRPLYSPROALATHRLYSTHR
11   ASPGLNVALILEGLNSERLEUILEALALEU
12   VALASNASPPROGLNPROGLUHISPROLEU
13   ARGALAASPLEUALAGLUGLUTYRSERLYS
14   ASPARGLYSLYSPHESERLYSASNALAGLU
15   GLUPHETHRLYSLYSTYRGLYGLULYSARG
16   PROVALASP

Samples:

sample: UbcH7, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS 25 mM; EDTA 1 mM; DTT 5 mM; sodium chloride 200 mM

Sample_Conditions_for_UbcH7: pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsampleisotropicSample_Conditions_for_UbcH7
2D 1H-13C HSQCsampleisotropicSample_Conditions_for_UbcH7
3D HCCH-TOCSYsampleisotropicSample_Conditions_for_UbcH7
3D CBCA(CO)NHsampleisotropicSample_Conditions_for_UbcH7
3D C(CO)NHsampleisotropicSample_Conditions_for_UbcH7
3D HNCOsampleisotropicSample_Conditions_for_UbcH7
3D HNCACBsampleisotropicSample_Conditions_for_UbcH7
3D H(CCO)NHsampleisotropicSample_Conditions_for_UbcH7

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAB24925 BAE38300 BAE39867 BAE87197 BAF83772
EMBL CAA04156 CAA10265 CAA63538 CAA65755 CAG30492
GB AAB36017 AAG17922 AAH53368 AAH77940 AAH93503
REF NP_001006180 NP_001071562 NP_001086444 NP_001127614 NP_001172062
SP P68036 P68037 Q3MHP1 Q5R5I4
TPG DAA20415
AlphaFold P68036 P68037 Q3MHP1 Q5R5I4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks