BMRB Entry 15421

Title:
1H and 15N Chemical Shift Assignments of Recombinant Agitoxin2
Deposition date:
2007-08-08
Original release date:
2009-10-12
Authors:
Anangi, Raveendra; Chuang, Woei-Jer
Citation:

Citation: Anangi, Raveendra; Koshy, Shyny; Huq, Redwan; Beeton, Christine; Chuang, Woei-Jer; King, Glenn. "Recombinant expression of margatoxin and agitoxin-2 in Pichia pastoris: an efficient method for production of KV1.3 channel blockers."  PLoS ONE 7, e52965-e52965 (2012).
PubMed: 23300835

Assembly members:

Assembly members:
Agitoxin2, polymer, 38 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: yellow scorpion   Taxonomy ID: 6883   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Leiurus quinquestriatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPICZalphaA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Agitoxin2: GVPINVSCTGSPQCIKPCKD AGMRFGKCMNRKCHCTPK

Data sets:
Data typeCount
15N chemical shifts37
1H chemical shifts241

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AgTX2, monomer1

Entities:

Entity 1, AgTX2, monomer 38 residues - Formula weight is not available

1   GLYVALPROILEASNVALSERCYSTHRGLY
2   SERPROGLNCYSILELYSPROCYSLYSASP
3   ALAGLYMETARGPHEGLYLYSCYSMETASN
4   ARGLYSCYSHISCYSTHRPROLYS

Samples:

sample_1-AgTX2: Agitoxin2 2.5 mM

sample_2-AgTX2: Agitoxin2, [U-98% 15N], 2.5 mM

sample_conditions_1: ionic strength: 0 M; pH: 4.6; pressure: 1 atm; temperature: 300 K

sample_conditions_2: ionic strength: 0 M; pH: 4.6; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_2-AgTX2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2-AgTX2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1-AgTX2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1-AgTX2isotropicsample_conditions_2
3D 1H-15N NOESYsample_1-AgTX2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1-AgTX2isotropicsample_conditions_1
2D DQF-COSYsample_2-AgTX2isotropicsample_conditions_1
3D HNHAsample_1-AgTX2isotropicsample_conditions_1

Software:

AURELIA v3.1, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PIR P46111
PDB
SP P46111 P46112
AlphaFold P46112 P46111

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks