BMRB Entry 15337

Title:
Solution NMR Structure of SO0334 from Shewanella Oneidensis. Northeast Structural Genomics Target SoR75
Deposition date:
2007-06-28
Original release date:
2019-01-09
Authors:
Tang, Yuefeng; Wang, Dongyan; Nwosu, Chioma; Cunningham, Kellie; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano
Citation:

Citation: Tang, Yuefeng; Wang, Dongyan; Nwosu, Chioma; Cunningham, Kellie; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano. "Solution Structure of SO0334 from Shewanella Oneidensis. Northeast Structural Genomics Consortium target SoR75"  .

Assembly members:

Assembly members:
SO0334, polymer, 78 residues, 8676.478 Da.

Natural source:

Natural source:   Common Name: Shewanella oneidensis   Taxonomy ID: 70863   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shewanella oneidensis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: BL21(DE3)+Magic   Vector: SoR75_21.1

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts360
15N chemical shifts76
1H chemical shifts587

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SO03341

Entities:

Entity 1, SO0334 78 residues - 8676.478 Da.

residue 71-78 represent a non-native purification tag (LEHHHHHH)

1   METARGVALPHEPROVALTYRALAPROLYS
2   LEUILEVALLYSHISALAARGILEPHELEU
3   THRGLYVALILETRPVALLYSASPLEUGLY
4   ARGLEUGLUPHEGLULYSGLYARGPHELEU
5   LEUPROARGLYSSERLEUPROLYSVALLYS
6   GLNALAILELEUGLULEUASNGLULEUILE
7   GLUALAGLNASNHISGLNTHRLYSTHRALA
8   LEUGLUHISHIS

Samples:

sample_1: SoR75, [U-100% 13C; U-100% 15N], 1.1 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%

sample_2: SoR75, [U-5% 13C; U-100% 15N], 0.95 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY_aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D NH(CO)CACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC_high resolutionsample_2isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection

AutoAssign v2.2.1, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

SPARKY v3.110, Goddard - data analysis, peak picking

AutoStruct v2.1.1, Huang, Tejero, Powers and Montelione - structure solution

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

X-PLOR NIH v2.11.2, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

PSVS v1.3, Bhattacharya and Montelione - data analysis

PDBStat v4.0, Tejero and Montelione - PDB analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

SWS Q8EJX2_SHEON

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks