BMRB Entry 15309

Title:
1H Chemical Shift Assignments for Cardiotoxin A5 from Naja Atra at Neutral pH and temperature of 318K
Deposition date:
2007-06-17
Original release date:
2008-02-28
Authors:
Tjong, Siu-Cin; Wu, Po-Long; Wang, Chang-Mao; Huang, Wei-Ning; Ho, Nan-Lu; Wu, Wen-Guey
Citation:

Citation: Tjong, Siu-Cin; Wu, Po-Long; Wang, Chang-Mao; Huang, Wei-Ning; Ho, Nan-Lu; Wu, Wen-Guey. "Role of glycosphingolipid conformational change in membrane pore forming activity of cobra cardiotoxin"  Biochemistry 46, 12111-12123 (2007).
PubMed: 17918958

Assembly members:

Assembly members:
CTX_A5, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chinese cobra   Taxonomy ID: 8656   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Naja atra

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Data typeCount
1H chemical shifts1073

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cardiotoxin A51

Entities:

Entity 1, cardiotoxin A5 62 residues - Formula weight is not available

1   LEULYSCYSHISASNTHRGLNLEUPROPHE
2   ILETYRLYSTHRCYSPROGLUGLYLYSASN
3   LEUCYSPHELYSALATHRLEULYSLYSPHE
4   PROLEULYSPHEPROVALLYSARGGLYCYS
5   ALAASPASNCYSPROLYSASNSERALALEU
6   LEULYSTYRVALCYSCYSSERTHRASPLYS
7   CYSASN

Samples:

CTX_A5_sample1: CTX A5 1 mM; phosphate buffer 10 mM

CTX_A5_in_DPC_micelle: CTX A5 1 mM; DPC, [U-2H], 50 mM; phosphate buffer 10 mM

CTX_A5_in_10%SGC_DPC_micelle: CTX A5 1 mM; SGC 5 mM; DPC, [U-2H], 45 mM; phosphate buffer 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYCTX_A5_sample1isotropicsample_conditions_1
2D 1H-1H TOCSYCTX_A5_sample1isotropicsample_conditions_1
2D 1H-1H NOESYCTX_A5_in_DPC_micelleisotropicsample_conditions_1
2D 1H-1H NOESYCTX_A5_in_10%SGC_DPC_micelleisotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
EMBL CAA63979 CAA90964 CAB50697
GB AAB01540 AAB03219 AAB03220 AAB18387 AAB36928
SP P62375 P62377 Q91126 Q91137 Q91996
AlphaFold P62375 P62377 Q91126 Q91137 Q91996