BMRB Entry 15305

Title:
1H Chemical Shift Assignments for Cardiotoxin A3 from Naja Atra at Neutral pH and temperature of 318K
Deposition date:
2007-06-15
Original release date:
2008-02-28
Authors:
Tjong, Siu-Cin; Wu, Po-Long; Wang, Chang-Mao; Huang, Wei-Ning; Ho, Nan-Lu; Wu, Wen-Guey
Citation:

Citation: Tjong, Siu-Cin; Wu, Po-Long; Wang, Chang-Mao; Huang, Wei-Ning; Ho, Nan-Lu; Wu, Wen-Guey. "Role of glycosphingolipid conformational change in membrane pore forming activity of cobra cardiotoxin"  Biochemistry 46, 12111-12123 (2007).
PubMed: 17918958

Assembly members:

Assembly members:
cardiotoxin_A3, polymer, 60 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chinese cobra   Taxonomy ID: 8656   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Naja atra

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Naja atra

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cardiotoxin_A3: LKCNKLVPLFYKTCPAGKNL CYKMFMVATPKVPVKRGCID VCPKSSLLVKYVCCNTDRCN

Data typeCount
1H chemical shifts1102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cardiotoxin A31

Entities:

Entity 1, cardiotoxin A3 60 residues - Formula weight is not available

1   LEULYSCYSASNLYSLEUVALPROLEUPHE
2   TYRLYSTHRCYSPROALAGLYLYSASNLEU
3   CYSTYRLYSMETPHEMETVALALATHRPRO
4   LYSVALPROVALLYSARGGLYCYSILEASP
5   VALCYSPROLYSSERSERLEULEUVALLYS
6   TYRVALCYSCYSASNTHRASPARGCYSASN

Samples:

CTX_A3: cardiotoxin A3 1 mM; phosphate buffer 10 mM

CTX_A3_in_SGC_DPC_micelle: cardiotoxin A3 1 mM; DPC, [U-2H], 45 mM; sulfatide (SGC) 5 mM; phosphate buffer 10 mM

CTX_A3__in_DPC_micelle: cardiotoxin A3 1 mM; DPC, [U-2H], 50 mM; phosphate buffer 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 318 K

sample_conditions_2: ionic strength: 19 mM; pH: 6.0; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYCTX_A3isotropicsample_conditions_1
2D 1H-1H TOCSYCTX_A3isotropicsample_conditions_1
2D 1H-1H NOESYCTX_A3_in_SGC_DPC_micelleisotropicsample_conditions_1
2D 1H-1H NOESYCTX_A3__in_DPC_micelleisotropicsample_conditions_2

Software:

xwinnmr, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
EMBL CAA07686 CAA90963 CAB42053 CAB42055
GB AAA49386 AAB01541 AAB18382 AAB18383 AAB25733
PIR JK0222
PRF 0406231A 2207174B
SP O93471 O93473 P60301 P60302 P60303
AlphaFold P60303 O93473 P60301 P60302 O93471