BMRB Entry 15276

Title:
1H, 13C and 15N resonance assignment of 6aJL2(R25G), a highly fibrillogenic lamdaVI light chain variable domain.
Deposition date:
2007-06-01
Original release date:
2007-09-17
Authors:
Gutierrez-Gonzalez, Luis; Muresanu, Lucia; del Pozo-Yauner, Luis; Sanchez, Rosalba; Guereca, Leopoldo; Becerril, Baltazar; Luecke, Christian
Citation:

Citation: Gutierrez-Gonzalez, Luis; Muresanu, Lucia; del Pozo-Yauner, Luis; Sanchez, Rosalba; Guereca, Leopoldo; Becerril, Baltazar; Lucke, Christian. "1H, 13C and 15N resonance assignment of 6aJL2(R25G), a highly fibrillogenic lamdaVI light chain variable domain."  Biomol. NMR Assignments 1, 159-161 (2007).
PubMed: 19636854

Assembly members:

Assembly members:
6aJL2, polymer, 111 residues, 11853.8 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET27b

Data sets:
Data typeCount
13C chemical shifts464
15N chemical shifts119
1H chemical shifts713

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
16aJL21

Entities:

Entity 1, 6aJL2 111 residues - 11853.8 Da.

Residue number 20 does not exist in this sequence numbering

1   ASNPHEMETLEUTHRGLNPROHISSERVAL
2   SERGLUSERPROGLYLYSTHRVALTHRILE
3   SERCYSTHRGLYSERSERGLYSERILEALA
4   SERASNTYRVALGLNTRPTYRGLNGLNARG
5   PROGLYSERSERPROTHRTHRVALILETYR
6   GLUASPASNGLNARGPROSERGLYVALPRO
7   ASPARGPHESERGLYSERILEASPSERSER
8   SERASNSERALASERLEUTHRILESERGLY
9   LEULYSTHRGLUASPGLUALAASPTYRTYR
10   CYSGLNSERTYRASPSERSERASNHISVAL
11   VALPHEGLYGLYGLYTHRLYSLEUTHRVAL
12   LEU

Samples:

sample_1: 6aJL2 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_2: 6aJL2, [U-95% 15N], 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_3: 6aJL2, [U-95% 13C; U-95% 15N], 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_4: 6aJL2 1.3 mM; sodium phosphate 25 mM; sodium azide 0.05%; D2O 100%

sample_conditions_1: ionic strength: 25 mM; pH: 5.2; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HTQCsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HNCACOsample_3isotropicsample_conditions_1
3D C(CO)NHsample_3isotropicsample_conditions_1
3D H(CCO)NHsample_3isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_3isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

AURELIA v3.1.6, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - data analysis

FELIX v2000, Accelrys Software Inc. - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 19798 19870
PDB
DBJ BAA19991 BAC01822 BAC01823 BAC01824 BAC01857
EMBL CAD43014 CAJ75494 CAJ75495 CAP74492 CAP74493
GB AAB33217 AAG24687 AAG24691 AAG47681 AAM46210

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks