BMRB Entry 15057

Title:
Solution Structrue of C-terminal Bromodomain of Brd4
Deposition date:
2006-11-28
Original release date:
2008-06-24
Authors:
Liu, Y.; Wu, J.; Shi, Y.
Citation:

Citation: Liu, Y.; Wang, X.; Zhang, J.; Huang, H.; Ding, B.; Wu, J.; Shi, Y.. "Structural basis and binding properties of the second bromodomain of Brd4 with acetylated histone tails"  Biochemistry 47, 6403-6417 (2008).
PubMed: 18500820

Assembly members:

Assembly members:
Bromodomain-containing_protein_4, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b(+)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts433
15N chemical shifts103
1H chemical shifts727

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Second bromodomain of Brd41

Entities:

Entity 1, Second bromodomain of Brd4 114 residues - Formula weight is not available

1   GLUGLNLEULYSCYSCYSSERGLYILELEU
2   LYSGLUMETPHEALALYSLYSHISALAALA
3   TYRALATRPPROPHETYRLYSPROVALASP
4   VALGLUALALEUGLYLEUHISASPTYRCYS
5   ASPILEILELYSHISPROMETASPMETSER
6   THRILELYSSERLYSLEUGLUALAARGGLU
7   TYRARGASPALAGLNGLUPHEGLYALAASP
8   VALARGLEUMETPHESERASNCYSTYRLYS
9   TYRASNPROPROASPHISGLUVALVALALA
10   METALAARGLYSLEUGLNASPVALPHEGLU
11   METARGPHEALALYSMETLEUGLUHISHIS
12   HISHISHISHIS

Samples:

sample: Bromodomain-containing protein 4, [U-13C; U-15N], 1.0 mM; phosphate buffer, none, 20 mM; NaCl, none, 50 mM; EDTA, none, 5 mM; D2O, none, 10 % v/v

sample_conditions_1: ionic strength: 70 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsampleisotropicsample_conditions_1
3D 15N-separated NOESYsampleisotropicsample_conditions_1
2D NOESYsampleisotropicsample_conditions_1
2D TOCSYsampleisotropicsample_conditions_1

Software:

NMRPipe v2.3, F.Delaglio - processing

SPARKY v3, T.D.Goddard and D.G.Kneller - data analysis

CSI v1.0, David S.Wishart - data analysis

Molmol v2K.2, Koradi - data analysis

CNS v1.1, A Brunger, P Adams, M Clore, P Gros, M Nilges and R Read - refinement

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
REF XP_006524755 XP_006524756 XP_008763395 XP_011244845 XP_011382411

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks