BMRB Entry 11599

Title:
NMR structure of eIF1
Deposition date:
2015-10-16
Original release date:
2016-10-20
Authors:
NAGATA, Takashi; OBAYASHI, Eiji; ASANO, Katsura
Citation:

Citation: NAGATA, Takashi; OBAYASHI, Eiji. "NMR structure of eIF1"  .

Assembly members:

Assembly members:
eIF1, polymer, 108 residues, 12330.247 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Data sets:
Data typeCount
13C chemical shifts473
15N chemical shifts107
1H chemical shifts738

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eIF11

Entities:

Entity 1, eIF1 108 residues - 12330.247 Da.

1   METSERILEGLUASNLEULYSSERPHEASP
2   PROPHEALAASPTHRGLYASPASPGLUTHR
3   ALATHRSERASNTYRILEHISILEARGILE
4   GLNGLNARGASNGLYARGLYSTHRLEUTHR
5   THRVALGLNGLYVALPROGLUGLUTYRASP
6   LEULYSARGILELEULYSVALLEULYSLYS
7   ASPPHEALACYSASNGLYASNILEVALLYS
8   ASPPROGLUMETGLYGLUILEILEGLNLEU
9   GLNGLYASPGLNARGALALYSVALCYSGLU
10   PHEMETILESERGLNLEUGLYLEUGLNLYS
11   LYSASNILELYSILEHISGLYPHE

Samples:

sample_1: eIF1, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; D2O, [U-2H], 5%; H2O 95%

sample_conditions_1: ionic strength: 170 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v2.0.17, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution

Kujira v0.984, Naohira Kobayashi - chemical shift assignment, data analysis, peak picking

NMRView, Johnson - collection, data analysis, peak picking

NMRDraw, Delaglio - processing

NMRPipe, Delaglio - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

GB AAA35131

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks